The enzyme of interest in this experiment is acid phosphatase, a digestive-enzyme that is ubiquitous in nature and while it is found in the blood, kidneys, liver etc. of …show more content…
The experimental results were obtained from the use of a standard curve for a known concentration of nitrophenol and an experimental reaction to test the velocity of the pure and extracted acid phosphatase. Also, the rate at which the nitrophenol formed at different substrate concentrations was tested and a Michaelis Menten and Lineweaver-Burk plot was made to determine the Vmax and Km. Based on previous research, it was expected that the velocity for the extracted enzyme would have a higher rate of reaction than the pure enzyme. Also, it was expected that as the concentration of substrate increased, the Michaelis Menten and the Lineweaver-Burk plot would show an increased