It is hypothesized that the small intestine will contract in normal RTS and adding EPI will cause a decrease in baseline tension, decrease in max total force and decrease in active force, while frequency will remain constant. It is also hypothesized that adding MCh will cause an increase baseline tension, decrease in active force and increase in max total force with frequency remaining constant. It is hypothesized that that adding ADP will cause a decrease baseline force, active force and max total force and will keep frequency equal to baseline. Lastly, it is hypothesized that submerging in CAF will decrease baseline tension, max total force and active force and lower frequency. Each hypothesis will be tested using Oryctolagua cuniculus small intestine in solution with normal RTS and the substances of test. The CAF will be tested in an isolated solution. These solutions will all be self-contained in a curvette, submergered in a water bath at
It is hypothesized that the small intestine will contract in normal RTS and adding EPI will cause a decrease in baseline tension, decrease in max total force and decrease in active force, while frequency will remain constant. It is also hypothesized that adding MCh will cause an increase baseline tension, decrease in active force and increase in max total force with frequency remaining constant. It is hypothesized that that adding ADP will cause a decrease baseline force, active force and max total force and will keep frequency equal to baseline. Lastly, it is hypothesized that submerging in CAF will decrease baseline tension, max total force and active force and lower frequency. Each hypothesis will be tested using Oryctolagua cuniculus small intestine in solution with normal RTS and the substances of test. The CAF will be tested in an isolated solution. These solutions will all be self-contained in a curvette, submergered in a water bath at