Methylated cytosines leads to the condensation of chromatin meaning that it may cause the chromatin to be more tight and packed so it will promote gene silencing since it will not allow the chromatin to be expressed; however acetylated histones will promote transcription and thus reversing the effect of the methylated cytosine, making this choice incorrect.
C. Methylated cytosines and deacetylated histones associated with relaxed chromatin and gene expression This choice is incorrect. Once again, the methylated cytosine is shown to cause the chromatin to condense, however in this case this is not favorable since it wants the gene to be expressed. Thus, the methylated cytosine is not associated with relaxed chromatin and gene expression; and on the other hand, the acetylated histones have seen to open up the chromatin structure and promote transcription, so since this choice has deacetylated histones it does the opposite, making this choice incorrect.
D. Unmethylated cytosines and acetylated histones are associated with relaxed chromatin and gene