Two colonies where then taken from this plate and placed into two tubes. These tubes where then taken and stored at a temperature of 37 degrees Celsius where it was able to grow overnight. After it was able to grow the plasmid DNA was isolated using Quick Lyse Minirep. The mixture was then mixed in order to resuspend the cells that had settled on the bottom and pipeted into a Quick Lyse Lysis. This tube was then Cetrifuged at 13,000 rpm and washed with a buffer. After being washed it was eluted with another buffer and set aside for the next part of the
Two colonies where then taken from this plate and placed into two tubes. These tubes where then taken and stored at a temperature of 37 degrees Celsius where it was able to grow overnight. After it was able to grow the plasmid DNA was isolated using Quick Lyse Minirep. The mixture was then mixed in order to resuspend the cells that had settled on the bottom and pipeted into a Quick Lyse Lysis. This tube was then Cetrifuged at 13,000 rpm and washed with a buffer. After being washed it was eluted with another buffer and set aside for the next part of the