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What type of analytical techniques is the Bradford Reagent?
What is it used to measure?
Spectroscopic
Measure concentration of protein in solution
The colourimetric assay is based on the absorbance shift of what dye?
Maximum absorbance of this dye occurs at what wavelength?
The absorbance of this dye depends on what?
Coomassie Brilliant Blue
595 nm
Amino acid composition of protein
The shift from red dye, to Coomassie Brilliant Blue, involves 3 stages, with the transition from stage 2 to 3 involving proteins.
State the 3 stages involved.
Cation doubly protonated
Neutral singly protonated
Anion unprotonated
The dye, Coomassive Brilliant Blue reacts mainly with arginine. What 5 amino acids does it react less well with?
What 2 types of proteins is it less accurate for?
Histidine, lysine, tyrosine, tryptophan and phenylalanine
Acidic and basic (need neutral)
Monoamine oxidases catalyse what?
Where are they found?
Oxidation of monoamines
Outer membrane of mitochondria
State 3 places where MAO-A is found.
Where is MAO-B found?
MAO-A: Liver, GI and placenta
MAO-B: Placenta
MAO is required for the inactivation of monoaminergic neurotransmitters. State 3 types of these.
Seratonin, melanonin and adrenaline
Complete this equation:
RCH2NH2 + H2O + O2 ---(MOA)--->? + ? + ?
What does a change in absorbance signify?
RCHO + NH3 + H2O2
Amine used, aldehyde produced
In what reaction pathway does PGK have a use?
Where is it found?
A deficiency is often X linked recessive. What 3 disorders are produced as a result?
What can over expression of PGK cause?
Glycolysis
Cytosol
Haemolytic anemia, mental disorder and myopathy
Gastric cancer
Complete the following stages in the PGK reaction:
?<--(PGK)(ATP->ADP)-->?<--(GADPH)(NADH+H+->NAD++Pi)-->?
3-Phosphoglycerate
1,3-bisphospho glycerate
Glyceraldehyde 3-Phosphate
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