Introduction:
The purpose of this experiment was to determine the rates of respiration within refrigerated and non-refrigerated Mung beans. Cellular respiration is the oxidation of glucose molecules (C6H12O6) to produce ATP, CO2, and H2O. The generalized/condensed version of this formula is: C6H12O6 6CO2 + 6H2O + ATP (Energy). The specimen’s speed at which it oxidized the glucose was being tested to determine its metabolic rate (metabolic rate- the rate at which it metabolized/oxidized the glucose molecules for energy). The hypothesis of group 2 was that the Mung Bean specimen would respire at a faster rate when held at room temperatures, and slower rates when held at refrigerated temperatures. It was determined upon development of the hypothesis that the dependent variable would be respiration rate, and the independent variable would be temperature.
Methods: …show more content…
This Sparkview program is designed to capture data concerning CO2 production over consistent intervals to be set up by the user. These intervals were then set to take readings every 30 seconds for 10 minutes. The readings taken were in parts per million, also abbreviated as ‘ppm’. This meant that for every one million portions of atmosphere within the container holding the sample a fraction of this amount was given by the instrument denoting the amount of the target substance being tested (CO2 ). The total CO2 production was determined by subtracting the minimum value (in ppm) from the maximum value (in ppm), and the Rate of respiration was determined by dividing this answer by the 600 seconds of the 10 minute duration. This, consequently, developed a minimally accurate rate of production of the target substance by the