The elution that corresponds to the alpha-ketoglutarate will be separated from the rest of the biomass. Once the alpha-ketoglutarate is isolated, it can undergo an acid-base titration in order to determine its concentration. An auto-titration will be in order to get the most accurate midpoint. The necessary calculations and data analysis will be done to determine the final concentration present in the biomass. This can then be compared for all of the groups: the experimental ΔglgC cyanobacterial mutants and the control group that consists of just ΔglgC …show more content…
SYK-6. The muconic acid and adipic acid can be separated using using HPLC. Once both acids are separated, the muconic acid can undergo an acid-base titration in order to determine its concentration. Auto-titrations will be done on both acids and the data will then be analyzed so that the final concentration of each acid can be determined.
Since the cell density might vary among the different groups of bacteria, the amount of products produced should be reported as a percent mass. The concentration of each product can be converted to the total mass present. The mass of the total biomass from each bacterial group should be known in order to determine the percent mass. The results can then be compared and a conclusion can be