Patients received a conditioning regimen which included total body irradiation (TBI), thiotepa, fludarabine and anti-thymocyte globulin (ATG) and no additional GVHD prophylaxis 31,32. Patients were stratified based on KIR phenotypes expressing recognition of HLA-C alleles; the first with KIR ligand incompatibility in the GvH direction (donor-versus-recipient NK cell alloreactivity), and the second without32. In the presence of alloreactive NK cells in prior studies, this group demonstrated disease control by eradication of leukemia cells in vivo that resulted in marked improvement of survival in AML patients, with 65% event-free survival at five years in KIR ligand mismatched cohort compared to 5% in non-alloreactive group. These results were particularly notable given advanced stage disease ( 3rd complete remission or relapse) with 85% of patients with KIR-ligand
Patients received a conditioning regimen which included total body irradiation (TBI), thiotepa, fludarabine and anti-thymocyte globulin (ATG) and no additional GVHD prophylaxis 31,32. Patients were stratified based on KIR phenotypes expressing recognition of HLA-C alleles; the first with KIR ligand incompatibility in the GvH direction (donor-versus-recipient NK cell alloreactivity), and the second without32. In the presence of alloreactive NK cells in prior studies, this group demonstrated disease control by eradication of leukemia cells in vivo that resulted in marked improvement of survival in AML patients, with 65% event-free survival at five years in KIR ligand mismatched cohort compared to 5% in non-alloreactive group. These results were particularly notable given advanced stage disease ( 3rd complete remission or relapse) with 85% of patients with KIR-ligand