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12 Cards in this Set
- Front
- Back
During transcription, how are RNA nucleotides added to the RNA transcript. |
They are added in the 3' end of the transcript. |
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What direction is the DNA template strand being transcribed?
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3' -> 5' |
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Can genes overlap? |
yes |
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What are spliceosomes made of? |
SnRNPs - SnRNA and proteins and proteins |
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What is amino acyl tRNA synthetase? |
enzymes that attach the correct amino acid to tRNA. ATP -> AMP + 2pi |
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Where is the mRNA binding site?
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At its 5' end. It binds to the 5' end of the mRNA. |
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What are the start and stop codons? |
start = AUG end = UAA,UAG,UGA |
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What is the function of the release factor in protein synthesis? |
Release factor binds to the end codon in the A site and promotes polypeptide removing from tRNA (in the P site) |
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What is non sense mutation? |
mutation resulting in early termination codon |
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What is a mis sense mutation? |
One amino acid is changed (substitution) |
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What kind of cell is used in cloning? |
mammary cells, they are totipotent (can give rise to all other cells) as they contain all the genetic information to make a clone. |
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How can cells protect themselves from mutation? |
Nuclease cuts off the damaged strand. DNA pol adds missing nucleotides and ligased together. |