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111 Cards in this Set
- Front
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Catalase positive ; Coagulase negative causes UTI in young sexually active females
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Staphylococcus saprophyticus
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Catalase positive ; Coagulase positive
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Staphylococcus aureus
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Gram positive cocci in clusters
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Staphylococcus
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Gram positive cocci in pairs or chains
Catalase negative |
Streptococcus
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Non-pathogenic organism that is catalase positive ; Coagulase negative Bacitracin sensitive
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Micrococcus
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Beta hemolytic creamy white colonies that ferments mannitol
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Staphylococcus auerus
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Catalase positive
White non-hemolytic colonies Coagulase negative |
Staphylococcus
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Group A beta-hemolytic wide zone
Bacitracin sensitive causes strept throat rheumatic fever and glomerulonephritis |
Streptoccus pyogenes
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Group B Beta-Hemolytic narrow zone Resistant to Bacitracin
normal flora of female genital tract |
Streptococcus agalactiae
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Diplococci lancet shaped
Alpha hemolytic cause pneumonia and meningitis |
Streptococcus pneumoniae
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Group A Beta hemolytic streptococci infection occurring in children who have throat infections or skin infections can cause
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acute glomerulonephritis
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Haemophilius can become immune to ampicillin because of
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beta lactamase
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Thioglycollate broth is stored at room temperature and in the dark to
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decrease absorption of O2 by the medium
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Salmonella vs. Shigella
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Salmonella has motility while shigella is nomotile
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Liquid fecal specimen from infant should be tested for
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Salmonella-
Shigella Campylobacter jejuni |
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enterohemorrhagic Escherichia Coli
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causes hemolytic uremic syndrome
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Enteropathogenic E.Coli
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causes infantile (infancy) diarrhea
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Enterotoxigenis E. Coli
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traveler's diarrhea
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Enterinvasive E. Coli
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inflammation of the intestine or dystentery; bloody diarrhea
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Enteroaggregative E. Coli
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causes watery diarrhea ; community acquired
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Grows Hemophilius and Neisseria
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Chocolate Agar
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Fastidious bacteria
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Neisseria speices
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Grows Gram positive cocci
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PEA and Cloumbia Colistin Nalidixic Acid
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Selective for Gram negative bacteria differential for lactose fermenting rods
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MAC agar
Eosin-Methylene Blue |
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Selective for N. gonorrheae and N. meningitidis
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Thayer-Martin Agar
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Selective for N. gonorrheae
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Jembec
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Selective for c. diphtheriae
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Cystine -Tellurite Blood and Tinsdale Agar
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Enrichment- used to demorstate dimorphism of fungi
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Brain heart infusion agar with blood
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To back up anaerobes
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Thioglycolate broth
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Selective for legionella species
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Buffered Charcoal yeast Extract
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Selective for Vibrio
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Thiosulfate Citrate-Bile Salts
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Agar that causes C. diphtheriae enhanced grouping and metachromatic granules
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Loeffler's Meduim
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Differential for Candida species
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Cornmeal agar with Tween 80
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Isolation and differation of C. neofarmans
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Birdseed agar
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If there is a deterioration of methicillin discs
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it can cause an increased growth of any organism
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ability to test microorganism's ability to ferment sugars and to produce hydrogen sulfide
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TSI Slant
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hydrogen sulfide (H2S) reacts with ferrous sulfate in the medium to form what visible black precipitate
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ferrous sulfide
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Sulfide producing bacteria
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Edwardsiella
Salmonella Proteus Citrobacter |
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In TSI, All lactose fermenters result in
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yellow slant/yellow butt (acid/acid reaction)
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In TSI non-lactose fermenters results in
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pink/yellow or yellow/yellow (if sucrose is fermented)
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blackening of the medium at the interface of butt and slant
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Salmonella species
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Failure to obtain anaerobiosis in anaerobe jar is due to
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the inactivation of the palladium- coated alumina catalyst pellets
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Which gram negative organism doesn't grow on MAC agar and is Oxidase postive, catalase postive, and non motile
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Pasteurella multicida
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Most frequently isolated non fermenting gram negative rod that is Beta-hemolytic oxidase postive catalase postive motile
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Pseudomonas aeruginosa
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Non-fermenting, Gram Negative Rods
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Acinetobacter
Pseudomonas aeruginosa Burkholderia Chryseobacterium Stenotrophomonas |
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Non fermenting, Gram Negative Rods that is Oxidase negative
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Acinetobacter
Stenotrophomonas |
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Non fermenting Gram Negative Rods that doesn't cause nosocomal infections
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Chryseobacterium
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Gram-Positive Bacilli (rods)
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Bacillus Norcardia
Corynebacterium Erysipelothrix Lactobacillus Listeria monocytogenes |
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Gram-Positive Cocci (circular)
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Stapylococcus
Steptococcus Enterococcus Micrococcus Leuconostoc |
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Enterobacteriacease
Oxidase negative H2S negative |
Morganella morganii
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types of Vibrio species
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Helicobacter pylori
Campylobacter jejuni V. cholerae V. vulnificus |
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Gram-Negative Cocci
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Neisseria
Neisseria gonorrhea Neisseria meningitis Moraxella |
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Gram-positive anaerobes
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Bifidobacterium
Actinomyces Clostridium Eubacterium Peptostreptococcus Propionibacterium |
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Gram-Negative anaerobes
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Bacteroides
Fusobacterium Porphyromonas Prevotella Veillonella |
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Used to prepare a slide from a plate culture of a dermatophyte (Fungus) for microscopic observation
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Lactophenol cotton blue
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Used to stain fungus
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Lactophenol cotton blue
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Potassium hydroxide
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hastens the decomposition process of soft tissues (both animal and human), leaving behind only bones
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Gram-positive bacteria have a
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thick mesh-like cell wall made of peptidoglycan
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Stain blue when stained with gram stain
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Gram-positive
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Gram-negative bacteria also have an additional outer membrane which contains
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lipids
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The gram-positive cell remains purple and the gram-negative cell loses its purple color
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Alcohol used in gram stain
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Histoplasma capsulatum
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has tuberculate macroconidia 8-14 microns
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enzyme that results in resistance to the beta-lactam antibiotics
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Beta-lactamase
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Beta-lactam antibiotics are
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inhibtors of cell wall synthesis
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Penicillins
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Beta-lactams
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liquefy the mucus
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N-acetyl-Lcysteine i
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Ureaplasma urealyticum needs
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Sterols to grow
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Used as a source of iron
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ferric pyrophosphate
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bacterial infection that commonly is transmitted through sexual activity
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Ureaplasma urealyticum
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All specimens that have a cyst state recovered form fecal specimen for examination should be
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stored at refrigerator temperature
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For quality control testing for DNAse production
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E.coli and Serratia marcescens can be used
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Throat or nasopharyngeal swabs submitted of anaerobic culture
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should be rejected
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Penicillin is an antibiotic that
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inhibits cell wall synthesis
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Latex Agglutination
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helps distinguish Group B Strep form other beta hemolytic streptococci
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used to differentiate Group D enterococci
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6.5% NaCl
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Group D streptococcus is known as
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Enterococcus
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Growth on Bile esculin medium can help identify
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Streptococcus D
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Bacitracin test is useful for
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differentiating β-hemolytic Group A streptococci from β-hemolytic non-Group A streptococ
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Streptococcus B can be distinguished by
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latex agglutination
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All gram negative cocci are
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oxidase positive
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Gram negative cocci that grows on Chocolate agar that is both maltose positive and glucose positive
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Neiseria meningitides
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Chocolate agar base containing cancomycin, colistin, nystatin and trimethoprim is known as
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modified Thayer-Martin Agar
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KV-Laked Blood
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slective for Bacteroides species
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Sodium bicarbonate and sodium citrate are components of
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JEMBEC system
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an enriched medium for the selective isolation and cultivation of Neisseria species.
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Modified Thayer-Martin (MTM II) Agar
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Agar is an enriched selective medium used for the isolation and cultivation of pathogenics Neisseria species enriched with Lysed horse blood, horse serum, dextrose and yeast dialysate
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NYC medium
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Anti-microbial dilution tests
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can detect varying degrees of organism sensitivity and resistance
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clinical resistance to penicillin correlates with
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beta-lactamase production in Neisseria meningitidis
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Universally susceptible to penicillin
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Streptococcus pyogenes
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first generation cepalosporins can be represented by
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cephalothin
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Selective for Camplobacter
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Skirrow Agar
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CIN Agar
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selective for Yersinia
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Selective for isolation of Salmonella from stool
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Bismuth sulfite
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Campylobacter fetus
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must be incubated in a mircoaerophilic environment for optimal recovery
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used as treatment against the three main bacterial causes of meningitis: Neisseria meningitidis, Streptococcus pneumoniae and Haemophilus influenzae
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chloramphenicol
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antibiotic, used to treat many types of bacterial infections, particularly those caused by Gram-negative organisms and not used for Neisseria gonorrhea
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gentamicin
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if the media contains Cycloheximide it will be comprised of which species of fungi
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cycloheximide
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Candida albicans vs. Crytococcus neoformans
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urease negative vs. pink urease positive
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Which fungi grows as brown colonies on niger seed agar?
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Crytococcus Neoformans
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Positive urease test that turns pink
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Cryptococcus species
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Beta lactamase testing
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Staph. Aureus as positve
H. Influenzae as negative |
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A cell culture line commonly used for the recovery of Chalmydia trachomatis for specimens
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Mc Coy's
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disease of the adrenal glands most commonly caused by the bacterium Neisseria meningitidis
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Water-Friderichsen syndrome
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Bacteroides fragilis is the most common
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gram negative anaerobes
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Relapsing fever in humans is caused by
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borrelia recurrentis
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used to monitor bactericidal activity during antimicrobic therapy in cases of endocarditis
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serum bactericidal (combines the interaction of the pathogen, the antimicrobial agent, and the patient.)
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occurs when drugs can interact in ways that enhance or magnify one or more effects, or side effects, of those drugs
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Sherris synergism
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Taken to determine how quickly it is cleared from the blood
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tolerance
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immuno diffusion technique that is a virulence test performed upon Corynebacterium diphtheriae
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Elek test
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In a disk diffusion susceptibility test, zones of a larger diameter would result if
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the disk is placed on the inoculated media and left at room temperature for an hour before incubation
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Egg yolk agar with lecithinase
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Clostridium peringens
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