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40 Cards in this Set
- Front
- Back
selective |
inhibits or allows growth of organisms |
|
differential |
allow growth of all organisms (color change) contains a sugar organisms can use |
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if organisms use sugar |
produce an acid> change inph> change in color |
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fermentation |
metabolism of sugar breakdown or utlization of food source |
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selective and differential media |
mannitol salt agar macckoney eosin-methylene blue |
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mannitol salt agar selective |
salt, only staphyloccocci |
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mannitol salt differential |
mannitol yellow color |
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ph in mannitol |
red |
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macckoney selective |
crystal violet inhbits gram + |
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macckoney differntial |
lactose fermentors red |
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macckoney ph |
netural red |
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eosin-methylene blue selective |
eosin y and methylene blue inhbits gram + |
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eosin-methylene blue differntial |
lactose ecoli-metallic green e-areogenes- thick pink |
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carbohydrates |
sugar food source |
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byproducts in carbohydrate fermentation |
co2 gas- air bubbles pyruiv acid - color change if ph indicator present |
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TSI test |
glucose, lactose, sucrose-yellow h2s produciton- black |
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tsi test method |
stab and streak stab butt and draw up from line of insertion and streak on top of slant |
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IMvic tests |
indole citrate |
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indole test |
test for presence of enzyme tryptophanase- breaks down tryphtophan SIM deep + cherry red - yellow or black |
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what do you add to indole test |
kovac's reagent 10 drops |
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citrate test |
simmons citrate agar slants +-blue agar - green |
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can organisms utilize citrate as energy source |
yes |
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h2s test |
SIM + black - clear |
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purpose of special purpose media |
1.isolation of bacteria from a mixed population of organisms 2. differentiation among closley related groups of bacteria 3. enumeration of bacteria in sewage and water and food and dairy 4. assay of naturally occuring substances like anitbotics, vitamins, products of industrial fermentation 5. characterization and identification of bacteria by their abilities to produce chemical changes |
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colliform bacilli |
e.coli produces greater quanities of acid from lactose can ferment lactose |
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dysentery, typhoid, and paratyphoid bacilli |
not lactose fermentors |
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anerobic |
without oxygen |
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aerobic |
with oxygen |
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do all microorganisms use pyruvic acid in the same way |
no |
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tsi is designed to differentiate among with groups of bacteria |
enterobacteriaceae |
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why is the acid-base indicator phenol red in a TSI |
detect carbohydrate fermentation |
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why does TSI contain thiosulfate |
substrate for h2s production |
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why 18 to 24 hours to view TSI |
make sure carbohydrate substrate have not been depleted and the degradation of peptones yielding alkaline end products has not taken place |
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enterobacteriaceae |
found in intestinal tract of humans and mammals pathogens- ocassional pathogens normal intestinal flora |
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tryphtophan |
essential amino acid (from food) |
|
citrate permenase |
facillitates transport of citrate in cells |
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citrate is apart of what cycle |
krebs |
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enzyme of citrate |
citrase converted to pyruvic acid and carbon dioxide |
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motility in hydrogen sulfide |
not restricted to line of incoluation |
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ferrous ammonium sulfate in h2s production |
indicator combining with the gas forming an insoluble black ferrous sulfide perticipate |