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64 Cards in this Set
- Front
- Back
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chlamydoconidia |
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pseudohyphae &blastoconidia |
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conidia |
spore |
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arthroconidia |
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Colony Morphology |
Candida albicans |
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Microscopic: – Pseudohyphae, some true hyphae – Clusters of round blastoconidia at the septa of pseudohyphae – Large, thick walled, usually single terminal chlamydospores
– Germ Tube positive – Urease negative (variable) |
Candida albicans
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Candida albicans |
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Candida albicans |
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Colony Morphology |
Candida tropicalis |
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Microscopic: – Pseudohyphae, some true hyphae – blastoconidia singly or in small groups
anywhere along pseudohyphae – Few tear drop shaped Chlamydospores may be present – Germ Tube negative – Urease negative |
Candida tropicalis
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Candida tropicalis |
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Candida tropicalis |
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Candida tropicalis |
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Infections in bloodstream,urogenital tract, normal flora• Colony Mor – Cream colored, sm yeast like -Microscopic: – Oval yeast cells w/ single terminal budding • No pseudohyphae
•Germ Tube neg – Urease neg |
Candida glabrata
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Candida (Torulopsis) glabrata |
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• normal flora |
Cryptococcus albidus |
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Cryptococcosis or normal flora • C: Flat or slightly heaped, shiny, moist, mucoid – blood agar, may show wrinkling at edges Micro – Yeast cells round, dark walled, budding – No hyphae
– Capsules may be – Bird seed agar / Caffeic Acid test Positive – Germ Tube negative – Urease pos– India ink for capsule |
Cryptococcus neoformans
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Cryptococcus sp. |
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Cryptococcus sp. |
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Cryptococcus neoformans produces |
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caffeic acid test for |
Cryptococcus neoformans |
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The API 20-C strip |
carbohydrate yeast id tests |
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– Disease in severely compromised hosts |
Geotrichum |
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Geotrichum candidum |
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Geotrichum sp. |
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C:Usually pink or coral, orange to red Soft, moist, may be mucoid
• Micro Budding cells are round to oval Rare pseudohyphae– Faint capsule maybe |
Rhodoturola
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Rhodotorula rubrum |
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Rhodotorula rubrum |
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– Disease in immuno-compromised hosts |
Trichosporon sp. |
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Colony Morphology |
Trichosporon sp. |
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Trichosporon sp. |
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Trichosporon beigellii |
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Trichosporon sp. |
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Colony Morph– Variable: “white fuzzy” – No hyphae Thick walled barrel shaped arthroconidia Arthroconidia alternate in the hyphae w/ |
Coccidioides immitis |
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Spherules are lg, round, thick walled; contain endospores – DNA Probes are available – Immuno-diffusion 4 exoantigen
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Coccidioides immitis
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Coccidioides immitis |
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Coccidioides immitis |
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Coccidioides immitis |
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Colony Morph – Variable: white, beige,
orange, pinkish. Granular, powdery or woolly. – Light reverse • Microscopic: – Coarse Septate branched hyphae, no conidiophores – Arthroconidia not swollen or thick walled. – Arthroconidia alternate in the hyphae w/ empty cells that break away |
Malbranchea sp.
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Malbranchea sp. |
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Malbranchia sp. |
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Colony Morph Initially yeast like, then white & colony, turning brown or tan w/ age – At 35 – 37, cream to tan, waxy yeast phase • Micro: – Septate hyphae w/ short or long conidiaphores Round or pear shaped conidia lollipop appearance Older cultures have thick walled chlamydoconidia Scedosporium apiospermum
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Blastomyces dermatitidis
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Blastomyces dermatitidis |
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Blastomyces dermatitidis |
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Blastomyces dermatitidis |
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Blastomyces dermatitidis |
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Blastomyces dermatitidis |
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The colony is cottony and powdery with a pale brown color. Mature growth occurred in 6 days on SDA. There was no growth on media containing cycloheximide. The organism did not convert to a yeast form when cultured at 37 |
Chrysosoporium sp. |
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Chrysosoporium sp. |
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Chrysosoporium sp. |
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• Colony Morph – White- brown, fine dense several wks, lg thick walled macroconidia form |
Histoplasma capsulatum |
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– Macroconidia can be confused w/ Sepedonium |
Histoplasma capsulatum |
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Histoplasma capsulatum |
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Histoplasma capsulatum |
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Histoplasma capsulatum |
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Histoplasma capsulatum |
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Colony Morph: White & waxy then becoming colony. Reverse is white – At 37, little or no growth. • Micro: Septate hyphae w/ single or branched conidiophores – Conidia are round, thick walled, & rough & knobby
– No microconidia formed, no yeast phase |
Sepedonium sp.
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Sepedonium sp. |
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Sepedonium sp. |
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Colony Morph: White heaped compact, |
Paracoccidiodes brasilienses |
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Paracoccidiodes brasilienses |
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Paracoccidiodes brasilienses |
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Paracoccidiodes immitis |
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Paracoccidiodes immitis |