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15 Cards in this Set
- Front
- Back
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What are the different parts in a FPLC device?
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Pump
dectector fraction collector injection valve column column inlet |
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What are chaperones
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proteins that assist with the folding of a protein
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what are common hosts for expressing LDH?
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microorganisms
cell cultures whole higher organisms |
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What does e. coli need to grow?
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oxygen
LB broth 37 degrees celcius they double every 20min glucose ph 7 |
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Where are the e. coli cells stored and why?
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in glycerol stocks this prevents lysis of the cells
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What is a multiple cloning site?
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a short segment of DNA which contains many restriction sites
This can be used to create transgenic organisms |
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What is a hybridoma?
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A cell that is produced in the laboratory from the fusion of an antibody-producing lymphocyte (B) cell and a nonantibody-producing cancer cell using polyethylene glycol
It produces a continuous supply of a specific monoclonal antibody |
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How are monocolnal antibodies froma hybridoma purified?
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With affinity chromatography
antibody selectively binds to Protein, so a high level of purity is obtained elution with a low pH buffer or a high salt elution buffer is used to recover purified antibody |
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What are advantages and disadvantages of purification and characterization of monoclonal antibodies from a hybridoma
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Disadvantage-costly
Advantage-has high affinity to the column |
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What is electrophoresis
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movement of charged particles in an electric field
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What kind of solution is used in electrophoresis?
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Agarose-for nucleic acids
Polyacrylamide-for proteins |
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What is the purpose of electrophoresis?
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to separate proteins based on molecular weight
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what is High Content Screening?
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Automated microscopy and image processing
automated cell biology method |
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What is high content screening used for?
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drug discovery
it uses living cells as tests for molecule discovery The technology is mainly used to determine whether a potential drug affects aspects of cell function involved in a disease |
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What are the different processes in high content screening?
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biology,chemistry, image analysis, data analysis
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