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63 Cards in this Set
- Front
- Back
Pathogenic
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disease causing
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Transformation
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change in geno/phenotype due to assimilation of external DNA by a cell (bacteria most easily take up random DNA and transform into pathogenic bacteria)
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Bacteriophages (phages)
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viruses that eat bacteria by injecting their DNA, transforming the bacteria, and causing it to produce more phages. made up of only protein and DNA.
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Nucleotide
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one sugar-phosphate backbone with one nitrogenous base.
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Chargaffs rules
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equivalent number of A=T
G=C |
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Double Helix
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2 strands of nucleotides connected by hydrogen bonds
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Purines
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adenine and guanine are twice as wide as their counterparts. (2 circles)
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Pyrimidine
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Cytosine and Thymine are only made up of one circle.
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Semi-conservative model
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model of DNA replication that represents one new and one old strand in each replicated strand of DNA. correct model.
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conservative model
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wrong model of DNA replication where the double helix seperates to give instructions to replicate other strands, and then forms again so that the original DNA strand is intact.
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Dispersive model
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Wrong DNA replication model where each strands contains mixture of old and new DNA.
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Origins of Replication
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sites at which the replication begins.
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Replication forks
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where the DNA strand is still intact, but the new DNA is working its way toward it. where new DNA strands elongate.
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DNA polymerase
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catalyzes elongagtion of the replication fork.
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Nucleotide triphosphate
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a nucleotide with 3 phosphates.
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leading strand
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new DNA strand going from 5' end to 3' end. going towards replication fork.
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lagging strand.
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New DNA strand going towards the origin of replication from 5' strand to 3' strand.
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Okazki fragments
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segments of lagging strand made one by one and connected later.
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DNA ligase
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joins sugar-phosphate backbone of Okazaki fragments, forming single, new DNA stand.
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Primer
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initial nucleotide chain of RNA.
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Primase
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Enzyme that starts RNA from scratch.
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Helicase
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enzyme that untwists helix at the origin of replication.
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Topoisomerase |
enzyme that relieves stress of untwisting that Helicase causes. This enzyme also works in bacteria DNA replication to seperate the two circles. it works mostly by seperating the DNA strands to swivel, move, or turn them as neccesary. |
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Single-strand binding protein |
Enzyme that binds and stabilizes unpaired DNA strands until synthesis can occur. (DNA is volatile and in danger when unpaired (not two strands connected)) |
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Mismatch Repair
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when an enzyme is needed to fix incorrectly paired nucleotides.
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Nuclease
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enzyme that takes out damaged DNA and replaces.
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Excises
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takes out bad DNA.
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Nucleotide Excision Repair
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DNA repair with Enzymes.
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Telomeres
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Not genes, just repetition of meaningless sequences to postpone erosion of genes (every time DNA strand gets shorter). may have a hand in the aging process associated with the shortening DNA strand and the losoing of Telomeres.
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Telomerase
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enzyme that lengthens Telomeres.
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DNA pol III (Polymerase)
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the protein enzyme that synthesizes leading strand/Okazki fragments in DNA replication.
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DNA pol I (Polymerase)
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removes primer and replaces RNA with DNA.
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Histome protein |
protein which DNA wraps itself around. |
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Frederik Griffith (1928)
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date and name of the person who conducted the first experiment involving mice and harmful pathogenic bacteria. he took harmful S and harmless R and found that dead S mixed with live R transformed the R into harmful bacteria that killed the mice.
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Transformation
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bacteria takes in DNA from enviroment and changes to suit that bacteria.
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Oswald Avery (1944)
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Date and name of the man who cunduced a secondary experiment with mice and harmful S and harmless R bacteria. he found that when he seperated all the components of the dead S bacteria, DNA was the only factor that transformed the harmless R bacteria.
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Transforming Agent
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DNA transforms and therefore is a ______.
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Streptococcus pneumoniae
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bacteria used by Avery and Griffith in their experiments with mice.
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Alfred Hershey and Martha Chase (1952) |
name and date - performed experiment with bacteriophages using radioactive sulfer to track the protein and radioactive phosphate to track the DNA and found that DNA was the only thing injected into the bacteria E. coli. |
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Bacteriophages (phages)
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made up only of protein and DNA. biruses that eat Bacteria by injecting DNA and causing the bacteria to produce more phages and eventually burst and release the phages to infect more bacteria.
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Irwin Chargaff (1937)
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date and name - discovered that the amount of Adenine roughly equaled the amount of Thymine and the amoung of Guanine equaled the amount of Cytosine.
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Base pair rules
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A=T
C=G |
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1953 - Watson and Crick.
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double helix is discovered.
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X Ray Chrystalography
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method of taking photos when they isolate/orientate molecules and photograph.
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Rosalind Franklin
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expert at Chrystalography and took picture that led to the discovery of the double helix.
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Antiparallel
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two strands of DNA go opposite directions.
5' - 3' 3' - 5' (DNA replicated/built from 5' end to 3' end) |
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Hydrogen bonds
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bonds between bases
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Adenine and Thymine
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have 2 hydrogen bonds connecting them between their strands.
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Guanine and Cytosine
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have 3 hydrogen bonds connecting them between the strands.
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Phosphodiester bonds |
covalent bond in backbone holding nucleotides together. |
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.34 nanometers
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distance between bases (steps of ladder)
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3.4 nanometers
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one complete turn of helix.
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1 nanometer
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distance between middle of helix (center) and outside.
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Deoxyribose
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Ribose without oxygen.
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Ribose
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Ribose (with normal oxygen)
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Nucleotide Tri Phosphate |
the new base being brought in with all 3 phosphate groups. |
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Circular Chromosomes
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chromosomes that are connected at both sides and are found only in Prokaryotic cells or mitochondria/chloroplasts.
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DNA clamp
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a newish discovery of the other protein that binds to DNA polymerase and keeps the polymerase stable.
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apopstasis
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cell is programmed to die, connected to telomerese running out.
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Thymine Dimers
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caused by UV rays and happens when two thymines next to each other bond to each other, causing a kink in the chain. (the thymines bulge out and the adenines on the other side come in). is fixed by an excision repair.
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DNA proofreading.
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when DNA polymerase fixes a mistake before moving on.
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Mismatch repair
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when a base was mispaired and missed in proofreading. can be fixed by nuclease excising area around mismatched pair and DNA polymerasecoming back.
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Excision Repair
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when DNA nuclease takes out "damaged" nucleotides and polymerase replaces them. damaged may mean mutated or harmed DNA, not neccesarily though replication.
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