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12 Cards in this Set
- Front
- Back
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What is the role of a primer in the PCR? |
enables replication/sequencing to start/keeps strands separate |
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What is the role of DNA polymerase in the PCR? |
joins DNA nucleotides |
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Suggest one use of the PCR? |
Replication of DNA from crime scene for DNA sequencing |
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2 ways in which PCR differs from transcription |
RNA polymerase/uracil PCR copies both strands |
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What is the role of a vector? |
Transfer genes from one organism into another |
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Descirbe the role of restriction endonucleases in the formation of plasmids that contain donor DNA? |
cut open plasmid cut out donor DNA both cut with same endonuclease sticky ends/bases exposed |
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Describe the role of DNA ligase? |
Annealing/backbones joined/phosphodiester |
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Explain why treatement by gene therapy in T cells must be repeated at regular intervals, whereas a bone marrow transplant = single cure? |
T cells die/dont reproduce bone marrow provides continous supply of lumphocytes/gene |
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suggest why ddnucleotides terminate sequencing? |
Don't react with nucleotides dont fit polymerase |
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In DNA sequencing, waht makes the DNA fragments visible on the autoradiograph? |
Radioactive PRIMER |
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Describe how genetic fingerprinting can be carried out on panda DNA |
DNA is cut using restriction enzymes (made single stranded) separated by electrophoresis southern blotting onto nylon membrane apply probe radioactive or flourescent autoradiography |
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Explain how scientists identify the father of a cub? |
All bands which dont come from mother must come from fathers DNA fragments |
