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11 Cards in this Set

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What are the three phases (4 if you count the transition reaction) of cellular respiration?
1. Glycolysis - breakdown of glucose (splitting of glucose into Pyruvate C3)

2. Transition Reaction - connects glycolysis to citric acid cycle

3. Krebs/Citric Acid Cycle - series of oxidation (The loss of electrons from a substance involved in a redox reaction.) (Creates 2 Acetyl (C2) and happens in the Mitochondria)

4. Electron Transport (Chains) Systems - carriers move electrons (happens in the Mitochondria)
What are the oxidation-reduction coenzymes? How do they function?
NADP+ = accepts 2 e- + H+ -->NADPH (photosynthesis)
NAD+ = accepts 2 e- + H+ -->NADH (cell respiration)
FAD = accepts 2 e- + 2 H+ --> FADH2 (cell respiration)

NADH & FADH2 carry e- to electron transport system

Coenzymes are small organic molecules that transport chemical groups from one enzyme to another.
What are the inputs and outputs of gycolysis?
Input - each glucose requires 2 ATP (-2 ATP)

Output - converts the Glucose to 2 Pyruvate (C3) and also gains 4 ATP and 2 NADH (+4 ATP & +2 NADH)
What are the inputs and outputs of the Krebs cycle?
Input - Converts pyruvate (C3) --> acetyl (C2)- coenzyme A (CoA) + C02
Acetyl CoA enters Krebs cycle in the matrix

Output - Cycle occurs in matrix of mitochondria (2 cycles)
Produces 4 CO2, 2 ATP, 6 NADH, 2 FADH2
NADH & FADH2 carry e- to electron transport system
Why do we call gylocolysis the energy investment phase?
Because in phase one it takes 2 ATP to activate the glucose (C6) and 1 Glucose to split into 2 Pyruvates (C3) and then produces a net of 2 ATP and 2 NADH
What is the basic mechanism at the electron transport system? (where are electrons going? Where are hydrogen ions going? How is that making ATP?)
Chain of electron acceptors in inner membrane of mitochondria

NADH & FADH2 carry electrons to electron transport system

Oxygen = final electron acceptor in chain (catches electrons & forms H2O)
O2 + 2 electrons + 2 H+ ---> H2O
Use O2 to form ATP from ADP = oxidative phosphorylation

Chemiosmosis: Hydrogen ions (H+) pumped across membrane to form a gradient = proton-motive force (mitochondria, chloroplasts & bacteria)


ATP synthase complex = protein complex in mitochondrial cristae
Flow of hydrogen ions down gradient = coupled to ATP production
Disrupted during cyanide poisoning & hibernation
What is the different between substrate level phosphorylation and oxidative phosphorlyation? In what phases of respiration do they happen?
Substrate-level phosphorylation = enzyme transfers phosphate from substrate to ADP-->ATP
This happens in the Glycoloysis and The Krebs Cycle (Citric Acid Cycle)

Oxidative phosphorlyation Uses O2 to form ATP from ADP
This happens in the Electron Transport System
What is the ATP synthase complex?
ATP synthase complex = protein complex in mitochondrial cristae

Flow of hydrogen ions down gradient = coupled to ATP production

This is disrupted during cyanide poisoning hibernation
What is fermentation?
A catabolic process that makes a limited amount of ATP from Glucose without an electron transport chain and that produces a characteristic end product, such as ethyl alcohol or lactic acid.

Anaerobic does NOT require O2

Glycolysis
Pyruvate reduced to:
lactate or
alcohol & CO2
What builds up when we run out of oxygen, and makes our muscles sore?
Lactic Acid
What are the different feedback mechanisms that control respiration?
When ATP or citrate = high
phosphofructokinase = inhibited
glycolysis slows down

When ATP = low & AMP levels rise:
phosphofructokinase = active
glycolysis speeds up