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20 Cards in this Set
- Front
- Back
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Why is yeast a good model for genetics studies? 6
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non-pathogenic
rapid growth cycle grow as dispersed cells easy to maintain in stable haploid and diploid states small genome size well defined genetic system |
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How does vegetative cell division of yeast occur?
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budding
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What are the 2 mating mating types that haploid yeast cells can exist?
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alpha mating type
a mating type |
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What do alpha and "a" mating types become when grown together?
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pear shaped called "shmoos"
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What do haploid yeast cells secrete?
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mating pheromones
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Pheromone?
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influences the behavior of another individual of the same species
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What do Shmmos mate to form?
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diploid zygotes
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Homothallic?
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wild yeast that rapidly change mating type during the haploid phase and then mate to form diploids
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Why are lab yeast Heterothallic?
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lab yeast strains are heterothallic due to a mutation in a gene that allows yeast to maintained as stably as eiyhrt diploid or haploid cells
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How can wild type yeast make its own adenine?
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from other food sources through the adenine biosynthesis pathway
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What do mutations in ADE1 or ADE2 cause?
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accumulation of the intermediate AIR which is converted to form the red pigment
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What does mutation in any of the genes earlier is the ADE patheay cause?
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block the pathway before the formation of AIR and prevent accumulation of pigment
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What can wild type yeast grow on?
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minimal media
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YED Medium?
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growth medium that contains yeast extract and dextrose
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YEPAD?
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very rich medium for storing yeast strains that contains yeast extract, peptone, adenine, and dextrose
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Auxotroph?
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mutants of yeast that are unable to synthesize an essential compound
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Prototroph?
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strain that grows on minimal media
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Why are haploid cells of yeast useful for complementation?
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the presence of defective allele for a gene in a metabolic pathway is directly observable at the phenotypic level as a defect in that pathway
-haploids shows their genotype |
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What are the requirements for complementation to take place in an yeast experiment?
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mating type
mutation in ADE pathway |
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Complementation test?
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used to decide if 2 recessive mutant phenotypes are determined by mutation in the same gene or 2 different
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