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90 Cards in this Set
- Front
- Back
FDA definition of red cell storage
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allows 75% survival 24 hrs after tx
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CPD storage time
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citrate-phosph-dextrose
21 days |
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CP2D storage time
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cit-phosph-dex-dex
21 days |
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CPDA-1 storage time
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+ adenosine
35 days |
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additive solutions
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as-1 adsol
as-2 nutricel as-3 optisol 42 days dex adenosine mannitol |
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additive solution RBC preparation
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CPD or CP2D, spun, then +110 mL additive (500 mL) or +100 (450 mL)
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HCT of additive solution RBC
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55-65%
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RBC storage
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42 days (additives) at 1-6 deg
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frozen RBC storage
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10 years @ -65
use 24 hours after thaw |
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washed RBC storage
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24 hours at 1-6 deg
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platelet storage
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5 days @ 20-24 C agitated
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pooled platelet storage
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4 hours
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granulocyte storage
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24 hours @ 20-24 deg, not agitated
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FFP storage
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1 year @ -18 C
7 year @ -65 C |
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thawed FFP storage
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24 hrs @ 20-24 C
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pooled FFP storage
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4 hours
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required RBC HCT
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< 80%
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required RBC Hb
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>50 g in 95%
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LR RBC required WBC
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< 5 x 10 ^6 in 95%
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LR RBC required retained RBC
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85%
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platelet # requirement
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> 5.5 x 10 ^10
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required platelet pH
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> 6.2 in 90%
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LR platelet required number
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> 5.5 x 10^10 in 75%
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LR platelet required pH
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> 6.2 in 90%
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LR platelet required WBC num
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<8.3 x 10^5 WBC in 95%
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apheresis platelet num require
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> 3.0 x 10^11
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apheresis platelet pH require
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pH > 6.2 in 90%
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LR apheresis platelet diff require
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< 5.0 x 10^6 residual in 95%
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CRYo requireemnts
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Factor VIII > 80 IU
fibrinogen > 150 mg |
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granulocyte conce requirement
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> 1.0 x 10^10 in 75%
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whole blood contents
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450-500 mL
RBC 200-250 mL plasma 250-300 mL WBC 10^9 platelets anticoags 63-70 mL |
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how fast can an effect of Tx be measured
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15 minutes
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RBC shipping T
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1-10 C
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compatible fluids RBC
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normosol-R, plasma-lyte
5% albumin NS plasma |
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incompatible fluids RBC
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LR, hypo or hypertonic saline
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rejuvenated RBC
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3 days after expiration CPD, CPDA-1 or until expiration AS-1 with rejuvesol (ATP, 2,3-DPG)
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WBD platelet volume
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40-60 mL
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WBD platelet WBC num
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usually ~10^7
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neonatal platelet dose
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10-15 mL / kg
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platelet indications for thrombocytopathy
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1. congenital
2. drug 3. bypass/ecmo 4. secondary for metabolic (CRF) |
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contraindications for platelets
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1. itp 2. hit 3. ttp 4. post-transfusion purpura
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expected platelet response
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20-30k rise following 1 hr
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platelet shipping temp
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20-24 C
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LR filter WBC reduction
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99.99% reduction (4 log)
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what % of original component must remain following LR
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85%
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washed red cell shelf life
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24 hours
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washed platelet shelf life
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4 hours
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NAT/NAIT
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neonatal autoimmune thrombocytopenia
maternal anti-HPA-1A 1st pregnancy 10-30% intercranial hemorrhage |
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NAT/NAIT treatment prebirth
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IVIg
washed & irradiated maternal (or ag negative) platelets |
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NAT/NAIT treatment postbirth
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washed & irradiated maternal or ag negative platelets
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cryopreservatives
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usually glycerol 40%
DMSO is poor, only for platelets |
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which cpd can be frozen
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up to 6 day shelf life
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cpda-1 shelf life to frozen
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6 days
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cp2d shelf life to frozen
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6 days
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additive soln shelf life to frozen
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to full 42 days
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how long can rbc be frozen
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10 years @ -65 c
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how long can plt be frozen
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2 years at -80 c
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irradiation dose
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2500 cGy = rad, at least 1500 cGy to all parts.
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idx for irradiation
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1. congenital t-cell def.
2. hsct 3. anti-T cell chemo 4. aplastic anemia 5. solid tumor c ext chemorads 6. intrauterine tx 7. neonatal exchange 8. hematol malig / HL 9. granulocyte Tx 10. first degree or HLA matched |
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irradiation expiry
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28 days after radiation OR normal expiry date, whatever is 1st.
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FFP volume
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200-250 mL
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FFP fibrinogen content
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400 mg
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vitamin K length to activity
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6-12 hrs
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bleeding pt with coumadin, dose
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10-20 mL/kg FFP
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expected FFP effect
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increases factor 20-30%
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FP24
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frozen within 24 hours, not 8
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FP24 factor def
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factor 8 is 25% less
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fp24 when can it be used
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anything as FFP but FFP is better for DIC
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thawed FFP good for
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24 hours
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thawed FP24 good for
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24 hours
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thawed plasma
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good for 5 days at 1-6 c
is basically thawed FFP/FP24 |
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liquid plasma
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plasma from blood 5 days expired
stored 1-6 c, not frozen can be tx up to 5 days following expiry |
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cryoprecipitate contents
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15 ml vol
150 mg fibrinogen 80 IU factor 8 100 IU vWF 50 IU factor 13 fibronectin |
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tx uremic thrombocyopathy
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cr > 3 mg/dL
1) DDAVP 2) dialysis 3) cryo adhesion defect |
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tx vwf with cryo
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1 bag / 10 kg q 8 hrs
if severe & no factor 8 cocnentrate |
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making cryo
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1 unit FFP thawed to 1-6 c
spin and remove liquid ppt within 24 hrs store -18 c @ 1 yr |
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pooled cryo exp
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tx within 4 hrs
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PCC
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prothrombin complex concetrate
hemophilia B or warfarin overdose (factor 9 complex concentrate) |
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PPF
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plasma protein fraction
83% albumin (vs 96% in albumin) |
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granulocyte concentrate contents
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200-300 mL volume
> 1.0 x 10^10 WBC 1 x 10^11 platelets plasma and anticoagulant |
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granulocyte tx background indications
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premature neonates with sepsis
transplant patients chronic granulomatous dz |
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situations for granulotcyte tx
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fever 24-48 hrs
proven infection no response to abx < 500/uL neutropenia reversible bone marrow hypoplasia |
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granulocytes: ta-gvhd and cmv safety
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cannot filter, must irradiate and use CMV-negative
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granulocyte storage
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24 hours within collection 20-24 c without agitation
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DDAVP action
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release of vwf/factor 8 from endothelial cells
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ddavp in uremic thrombocytopathy
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0.3 ug/kg IV
BEFORE platelets or cryo |
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uses of ddavp
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1) uremic thrombocytopathy 2) vwf 3) mild hemo A 4) hepatic failure
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chronic ddavp use
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tachyphylaxis
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novoseven uses
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1) hemo A/B inhibitors 2) congenital factor 7 deficiency
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half life novoseven
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2 hrs
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