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40 Cards in this Set

  • Front
  • Back

Size of prokaryotes

0.5-5 micrometers

Plasmid characteristics

Virulence


Resistance to antibiotics


Bacteriocin(natural antibiotic)


Fertility(transferred in conjunction)

Bacterophages

Viruses that can infect gram negative bacteria reacting with specific proteins on cell surface

Viroids

Circular ssRNA loops with no capsid protein coat

Prions

Infectious proteins


Occur spontaneously from mutation leading to a misfolded protein and a loss of the 3D structure

How to Prions act on cells?

Build up inside cell and poison it causing it to lyse and release the prions into the space around it


can lead to degenerative diseases like mad cow disease

Prophage

When the bacteriophage genome is inserted and integrated into the host DNA.


NB it circulizes first

How does the change occur from the Lysogenic to the lytic cycle?

Environmental or chemical triggers cause the release of the prophage from DNA leading it to control cell by degrading the host DNA.

Reverse transcriptase

Viral enzyme that sytheszies complementary DNA strands to viral RNA

Viral envelope

A lipid bilayer gained from plasma membrane when envelope type viruses leave their host cell


Has surface glycoproteins allowing it to stay 'hidden' in the body whilst being specific for its host range

Provirus

Virus genome that is integrated into the DNA of a host cell

Size of nucleus

5-10 micrometers

Nuclear envelope

2 lipid bilayers

Nuclear lamina


4

Lines inner surface of nuclear envelope


Intermediate filaments


Helps maintain shape of nucleus


Also helps organise DNApacking

Nuclear Pore Complex


4

Highly regulated


Allows specific substances in and out


RNAs allowed out


control signals, materials and energy allowed in

How is DNA packed?

The DNA double helix is combined with histone proteins to form chromatin fibres that undergo multilevel packing

First stage of DNA packing

Double helix(d=2nm) interacts with histone proteins(H2-H4) forming chromatin fibers with nucleosomes(d=10nm)

Nucleosomes

Bead like structures on chromatin fiberss where Histone proteins 2-4 are attached to the double helix


diameter of 10nm

2nd stage of DNA packing

Further interactions between DNA and histone 1 cause the fiber to coil forming a 30nm fiber

How big is the fibre caused by further interactions of DNA and histones? What histone is involved here?

30nm


Histone 1

3rd stage of DNA packing

The fibre the loops and is coiled more forming a 300nm fibre


During cell division these coil more forming metaphase chromosomes(very dense visible form)

Euchromatin

Less dense area of chromatin


More accessible


More active - contains useful genes at present

Heterochromatin

More dense area of chromatin


Less accessible


Less active aka genetically inactive

What is the relationship between euchromatin and heterochromatin?

It is a dynamic relationship to suit cells needs as the genes needed change over time to suit cells demand.

Chromosome location in nucleus is:

Not random


Chromosomes are anchored in place

What resists compression in the ECM?

Proteoglycans

What can scanning electron microscopes view?

Outer layers of cell

What can transmission electron microscopes view?

internal ultrastructure of cell

Advantage of small cell size:

A small cell has a large surface area relative to cytoplasmic volume

Endospores

Produced in adverse conditions.


Heat, chemical and desiccation resistant

Cholesterol

Increases membrane fluidity at moderate Temps


Decreases membrane fluidity at low Temps

Fibronectins

Glycoprotein


attach cells to ECM

Integrins

Membrane receptor protein bound to microfilaments.


Information changes in outside can be exchanged through a mechanical signaling pathway involving fibronectins, integrins and the cytoskeleton

Microtubules

Tubulin subunits


Resist compression


Organelle and whole cell motility


Hav e a central organization centre - centrosome

Microfilaments

Double chain of actin forming spiraling intertwined strands


Resists tension


Maintains cell shape


Involved in muscle contraction


Cytoplasmic streaming


Cell division

Intermediate filaments

Supercoiled fibrous proteins of keratin family


Anchor nucleus


Make up nuclear lamina


Reinforce cell shape

Cytoskeleton

GIves mechanical support to cell and maintains or changes its shape


Anchors or directs organelle movement


Controls cilia, pseudopod movement(sometimes muscular contraction)


Mechanically transmits signals from cells surface to interior


Interacts with motor neurons(proteins) that change shape to produce cellular movement

Resolution

Minimum distance that can separate 2 points that still remain identifiable as separate when viewed under a microscope

Magnification

Ratio of an objects image size to its actual size

Sexual Selection

Individuals with certain inherited characteristics are more likely than other individuals to obtain mates