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9 Cards in this Set
- Front
- Back
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USE OF MEDIA |
Isolation and Identification of Pseudomonas spp Also determine ability to produce pigments |
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OTHER NAME OF MEDIA |
Pseudomonas agar
Pseudo F agar King's B medium Modified Tech agar |
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MEDIA COMPOSITION
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Peptone -20g
MgCl2 - 1.4g Potassium Chloride-10g Cetrimide - 0.3g Glycerol - 10ml Agar -15g |
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SELECTIVE AGENT AND IT'S ACTION
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The selective agent is CETRIMIDE.
Cetrimide is an antiseptic which is mixture of different quaternary ammonium salts including Cetrimonium bromide . Cetrimide acts as a quaternary ammonium cationicdetergent causing nitrogen and phosphorous to be released from bacterial cells other than Pseudomonasaeruginosa. It inhibits all the other microorganisms |
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ACTION OF OTHER INGREDIENTS |
Gelatin- Nitrogen, Vitamins and Carbon source.
MgCl2 and Potassium Chloride - Enhance pigment production . Agar - solidifying agent. Glycerol - supplemented as a source of carbon |
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INCUBATION CONDITION |
Incubate the plates at 37 °C for 18 - 24 hr |
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CULTURAL RESPONSE
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Blue(pyocyanin) , or Yellow -green (fluorescein ) pigment which is seen with naked eyes . some times UV illumination is used to analyse pigment(Florescence) when organism doesn't show pigmented colony. |
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EXPECTED RESULT (for pyocyanin pigment) |
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EXPECTED RESULT
(for fluoresceinpigment) |
For more information microamaze.blogspot.com |
