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25 Cards in this Set
- Front
- Back
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limitations of leukocyte examinations |
Collecting Errors -Dilution Errors -Counting Errors |
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Causes of Hemolysis |
Agitaion of specimen -Forcible expulsion of blood -wet syringe -vacuum in the syringe -other chemical substance |
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Stains of blood smear |
Romanowsky -Wright's stain -Giemsa stain |
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Improve accuracy of estimation |
Electronic method |
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Performing total leukocyte count |
-Charging the counting chamber -counting the total leukocytes |
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Disadvantage of EDTA |
PCV decrease if excess -cytoplasmic vacuoles in leukemic neitrophils |
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Counting errors eumeration |
-improper preparation of blood smear -poor staining technique -Improper lighting aof microscope -inadequate exam of microscopic fields -inaccurate counting and cell enumeration |
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5 middle squares in the middle squares is used for |
Total erythrocyte count (RBC) |
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2ml of what in 100 ml distilled water added with 1 ml 1% Gentian violet |
Glacial acetic acid |
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HCL is prepared by adding ____ ml of concentrated HCL to ____ ml of h20
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1ml : 100ml |
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Center square is divided to ____ square measuring ____ sqmm and further subdivided in ____ small square measuring _____sqmm |
25 : 0.20 : 16 : 0.05 |
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4 corner squares are divided into _____ intermediate squares measuring _____sqmm
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16 : 0.25 |
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Which part of hemocytomeyer is used for total leukocyte count |
4 corner squares |
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square measuring 3x3mm divided into 9 equal squares measuring 1sqmm |
Neubauer ring |
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On each side of platform of each hemocytometer, an elevated glass support allows _____mm distance between the cover sleep and the ruled area |
0.1 |
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2 elevated platforms etched with neubauer ruling each platforms are surrounded by moat |
Hemocytometer
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Hemocytometer Method or material
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-Hemocytometer -special cover slip -Dilluting pipette -Microscpoe -Tally counter |
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Methods of total leukocyte count |
-Hemocytometer method -Electronic method |
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Prevents conversion of prothrombin to thrombin |
Heparin |
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Syringe rinsed with 1% ____ can preserve 5% blood |
Heparin |
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Leukocytic degeneration after 1 hr |
Oxalates |
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Give 3 examples of anti coagulant
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-heparin -Oxalate -EDTA (Ethylinediaminetetraacetic acid) |
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EDTA powder |
1mg per 1ml of blood |
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EDTA liquid
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1 drop of 10% sol. to 5ml of blood |
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Importance of leukocyte counting |
Confirming Diagnosis -Making a prognosis -selecting of the proper treatment |
