Enterobacteriaceae

Gram negative straight rods or coccobacilli with rounded ends

Test for Enterobacteriaceae

All members are with motile peritrichous flagella except;

All are no encapsulated except

Some organisms may grow in a low temperatures 1-5 C

At 37°Celcius it considered Non motile are

It ferments glucose and often gas production aerogenic except:

It catalase positive except;

All can reduce nitrate to nitrites except:

It is cytochrome oxidase negative except:

New members of Enterobacteriaceae

K or Capsular antigen Associated organisms

It posses V antigen

It is usually found among motile organisms

Protein in nature

Heat labile

2 types of enterics Based on clinical infections produce

Intestinal pathogens that not a normal flora

They generaly do not initiates disease

Opportunistic pathogens associated organisms

4 enrichment media that use to produce a number of bacteria

It is enrichment and selective media for Yersinia

Desoxycholate and Citrate salt as inhibitory

Mannitol as fermentable carbohydrates

It enhances the recovery of enteric pathogen from feces specimens (Salmonella &Shigella)

It is the ability of organisms to produce acid (acid formation)

Use to dispense as slant and butt

Acid pH result color

Alkaline pH result remain color

Lactose fermenter organisms

Late lactose fermenter organisms

Non lactose fermenter organisms

It contains glucose and lactose as it's fermentable carbohydrates

It is primarily use to differentiate Lactose fermenter

It is base on the ability of organisms to produce indole from tryptophan. It detects tryptophanase

Indicator of "indole test"

Indole test positive results

If negative result

It is based on mix acid fermentation pathway that organisms used glucose fermentation

Methyl Red Test indicator

Methyl Red Test positive results

Based on butylene glycol pathway that organisms used for glucose fermentation.

Detects acetoin or acetylmethylcarbinol

Vogues Proskauer Test positive result

It is based on the ability of organisms to utilize citrate as a sole source of carbon

Citrate Utilization Test positive results

Citrate utilization test indicator

It is useful in differentiating Salmonella (H2S +) from Shigella (H2S -)

Most sensitive for H2S production

H2S indicator

H2S positive organisms

H2S negative color

H2S positive color

It is useful in the identification of motile from non motile , between Klebsiella and Shigella, the only non motile coliforms

Motility Agar used for the detection of bacterial motility.

It is useful in identification of PPM based on the conversion of urea to ammonia through the action of urease.

Rapid Urease Producers (RUPo)

positive result with 4 hours

Slow Urease Producers (SUPo)

Positive after 4 hours

Urease Reaction broth use

Christensen's urea Agar or Stuart urea broth indicator

3 Ureas reaction positive results

Organisms that is Deaminase positive

It is use for the detection of Proteus, Providencia, Morganella only Deaminase +

It is use to detect lactose fermenter .

It is use to differentiate CITROBACTER(+) from SALMONELLA(-)

O-Nitrophenyl- Beta-D-Galactopyranoside) (ONPG test) positive result

ONPG positive

ONPG (-)

It detects the ability of organisms to remove the carboxy group from a specific amino acid

Culture media(broth) use of Decarboxylation reaction

It detects the deamination and decarboxylation, detect H2S production

Organisms that reported as R/A

(Red slant/acid butt )

Organisms that reported as K/A

(Alkaline slant & acid butt)

Organisms that reported as K/K

(Alkaline slant/alkaline butt)

Gelatin hydrolysis positive bacteria

Positive result in gelatin hydrolysis

It use to differentiate salmonella(+) from Shigella (-)

Malonate utilization indicator

Malonate utilization positive result, and negative result

It is a primary marker of fecal contamination. A normal flora of gastrointestinal tract

It cause cystitis an infection of urinary bladder

It is the #1 cause of UTI infection

#2 cause of neonatal meningitis

-It is most common cause of Turista) Travelers diarrhea or Montezuma's Revenge

-Characterize by profuse water diarrhea ,cholera like

-Produced heat labile, and or heat stabile enterotoxin

It causes Shigella like toxin infection

-it invades the intestinal epithelium that causes bleeding of the GIT

-stool sample: bloody stool

-It causes infantile diarrhea (in infant)

-does no produced toxin

-Nosocomial: hospital acquired

-Non invasive

It is most clinically significant (80% death)

-there is a production of Verotoxin

-Most severe manifestation of HEMOLYTIC UREMIC SYNDROME with serotype EHEC 0157:H7

agar used in the detection of E. coli O157:H7.

All Escherichia coli ferments sorbitol + color

It is the only non-sorbitol fermenter color is Yellow/brown colonies on SMAC

It produces Shigella toxin instead of Verotoxin

It is characterized by the presence of

Azotemia, low blood pressure, shock, low platelets count, and death

The purpose of the test is for the detection of Escherichia coli from water or food samples

Positive resulf of MUG test

It is a test use to indentify Enteroinvasive E. coli and Shigella spp.

It resembles Escherichia coli but are lactose negative

It causes bacillary dysentery/shigellosis

It is also known as Shiga bacillus

Also known as strong bacillus

Also known as New Castle Manchester Bacillus

Also known as Duval's bacillus

It is previously known as "Bethesda ballerup"

It highly resembles Salmonella

It causes sepsis and human infection

documented to cause nursery outbreak of neonatal meningitis in the USA

It is identified as a source of GI infection

Biochemically resembles Escherichia coli but NLF and H2S +

IMVic reaction (++--)

It produces gas(large amount of carbohydrates fermentation test-Aerogenic)

-Have the same biochemical characteristics

(TRIBE GROUP)

It is also known as "Friedlander's bacillus"

Homogeneous pink colonies on MAC

Another presumptive examination for identification of klebsiella pneumoniae

Reagent use K. pneumoniae

It is known to cause purulent sinus infection and foul smelling

Known to cause granuloma of nose and face

It leads to the malformation of face and neck

Previously know as Enterobacter alvei

Most important characteristic is delayed citrate

It has same biochemical characteristics with Serratia

A producer of different enzymes:DNase(+), lipase (+)Gelatinase (+)

A slow lactate fermenter

It produces red pigment PRODIGIOSIN when incubated at room temperature

Rare agent of human infection

Produces a rancid potato like odor

To differentiate Hafnia from Serratia perform

Suspected if the TSI is yellow over orange due to weak acid production in slant an no change in butt

Also know as Plague bacillus

3 types of antigenic determinants

O or somatic antigen what organisms present

It is known to cause Necrotizing colitis due to powdered milk formula

New name of Enterobacter sakazakii