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121 Cards in this Set
- Front
- Back
TP/TP+FN= ? TN/TN+FP=? TP/TP+FP=? |
SENSITIVITY SPECIFICITY PRECISION |
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What might the following indicate?urine: RBCs, WBCs, nitrite, bacteria |
PYELONEPHRITIS |
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Why is albumin the first protein to be detected in tests for renal failure? |
ITS MOLECULAR SIZE IS SMALLEST |
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Cortisol excess will result in _____
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EXCESS GLUCOSE IN THE BLOOD |
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This spiral-form organism is seen in urine and cultured on Fletcher’s mediaa. Borreliab. Leptospira |
Leptospira |
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Organism that gives off a bleach-like odor in culture? |
EIKENELLA |
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Procedure #1 detected 50/100 true positives and 100/100 true negativesProcedure #2 detected 80/100 true positives and 70/100 true negatives
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PROCEDURE 2 IS MORE SENSITIVE |
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What is the reason for the following discrepancy?Front TypePatient cells vs anti-A demonstrate a reaction strength of 3+Patient cells vs anti-B demonstrate a reaction strength of 3+Back TypePatient serum vs reagent A cells demonstrate a reaction strength of 3+Patient serum vs reagent B cells demonstrate a reaction strength of 0 |
THE PATIENT MAY BE A2B. |
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Presence of rheumatoid factor in blood may result in false positives for what test |
VDRL. |
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Disease associated with the following results? Elevated TSH; Elevated T3; Elevated free T4 |
PITUITARY TUMOR |
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If excess PTH is released, what would you find in elevated amounts in serum |
Calcium |
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Mucoid, pink colonies on plate; produces gas; indole (+). On TSI tube you see yellow on the slant and yellow in the deep. What organism is this? |
Klebsiella Pneumoniae |
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PAD (+); indole (+); Organism stains gram negative. What is it? |
P.vulgaris |
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You see a curved gram negative bacilli. It was cultured from the GI tract of a person with ulcers. What test would you do next to confirm its identity? |
TEST FOR UREASE |
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Enzyme controls run on a machine give results around -3 standard deviations. Samples run on the same machine give results of less than 1 standard deviation. What could be the problem? |
CONTROLS WERE LEFT AT ROOM T FOR SEVERAL DAYS |
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HIV-1 & HIV-2 combination ELISA test is positive in a patient with symptoms of immune deficiency. Western blot was inconclusive for HIV-1. What do you do next? |
DO HIV 2 WESTERN BLOT |
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What are the steps of PCR? |
denaturation, annealing, transcription |
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RAST test detects what?
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IgE |
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After collecting a blood sample in an EDTA tube for CBC, you find that the Hematocrit is very high (67%). What should you do next? |
REPORT THE RESULTS |
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When you conduct a procedure using fluorescence, it’s important to protect yourself from the |
EXCITING LIGHT |
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Blood was collected on Nov 1. Blood was then frozen in glycerol on Nov 5. What should the expiration date read? |
c. Nov 1; 10 years from now
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22. A person was successfully treated for syphilis 12 years ago. However, he has just come in again, worried about having been re-infected. What would you look for in his blood? |
VDRL |
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A patient demonstrates a positive antibody screen. You suspect either Jka, K or c antibodies. You know from a previous history that this patient has Jka antigen on their red cells. You then react the patients serum with cells positive for certain antigens and see the following:Patient serum vs: reagent K cells reagent c cellsReaction strength: 0 4+What can you conclude about the antigenic makeup of this patients red cells?
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d. rule out c antigen but cannot confirm the presence or absence of K antigen on the patients red cells |
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Urine protein chemistry dipstick (Reagent strip) detected no proteins but sulfosalicylic acid (SSA) test did detect proteins. Why? |
Bence-Jones proteins in urine |
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Syndrome of inappropriate antidiuretic hormone secretion (SIADH) would result in what in blood? |
deficient sodium |
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Fiber strands in urine resemble what under the microscope? |
Hyaline Cast |
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Which of the following regulates myocyte contraction? |
TROPONIN |
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HBa1c levels cannot always be used to monitor glucose levels in conditions such as |
SICKLE CELL DISEASE |
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What RBC inclusion can be seen on blood smear of a child who accidentally ingested moth balls? |
HEINZ BODIES |
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Which of the following parasite cause autoinfection in immunocompromised px? |
S.stercoralis |
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Which of the following causes antibody against TSH? |
Hashimotos Thyroiditis |
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Which of the following Mycobacteria we can acquire from tap water? |
M. Gordonae |
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Which of the following analytes is cofactor for most of 300 enzymes? |
Zinc |
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Pigment found in the lungs of cystic fibrosis patients is called? |
Pyocyanin/Pyoverdin |
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Specimen for Rotavirus? |
Stool |
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Menstruation results in the decrease of what? |
Ferritin |
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Most common cause of sperm agglutination is? |
Presence of sperm antibodies |
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Which value is increased in mumps? |
Amylase |
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What is a transudate and exudate? |
Transudate= Extravascular fluid with low protein content. Exudate= Exudate is fluid that leaks around the cells of the capillaries caused by inflammation. High protein content. |
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Define Blastoconidia? |
Budding of a yeast cell. |
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Malassezia Furfur? |
OLIVE OIL |
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If a person undergoes salicylate overdose what would you test for? |
pH DECREASE. METABOLIC ACIDOSIS. |
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ALP PH= 9.6 WHAT DISEASE IS PRESENT? |
PAGETS. |
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Cocaine metabolite? |
Benzoylecgonine |
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Which cells release heparin/histamine? |
Basophils and mast cells. |
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Positive RPR negative FTA for syphillis |
FALSE POSITIVE |
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Alpha Thalassemia is made up of? |
Hgb Bart and Hgb H |
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Eosinophils in the Urine are seen in what condition? |
Interstitial Nephritis |
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Isoagglutinin of Type O? |
ANTI A B AND AB |
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HTLV confirmatory test is? |
Western Blot |
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Latex agglutination for S. aureus factors seen? |
Protein A and clumping factor |
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Potassium permanganate in auramine-rhodamine stain for Myco is what?
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Quenching Agent |
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Iatrogenic anemia is caused by? |
Multiple blood draws |
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RBC count very low and retic % is 0.1 what is this a hallmark of?
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Pure red cell aplasia |
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HgbA1C values would be decreased in |
Hemolytic Anemia |
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Lupus anticoagulant causes what? |
Increased risk of thrombosis |
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Sample taken from indwelling catheter. Patient isn’t on any anticoagulants yet PTT and TT are way elevated. Cause? |
Heparin |
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In which case is MG monitored? |
Eclampsia |
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Patient taking primidone showing toxicity, but blood levels normal. What do you do next? |
Test phenobarbital level. |
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Sperm count can be done on semen sample when? |
When liquefaction is complete |
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Rouleaux is undetectable at what phase? |
AHG |
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Sezary cells are associated with what cells? |
T-cells. |
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Conns syndrome is characterized by an increase in? |
Aldosterone |
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Virus transported for 92 hours or something = ? |
LYOPHILIZED |
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Gram neg cocci present after jaw surgery |
Veillonella |
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Micrcoccus is resistant to what? |
Furazolidone |
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Procainamide metabolite is? |
NAPA |
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Alpha thalassemia is made up of? |
HGB Bart/Major |
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Blood product with highest capacity to transmit hepatitis? |
Packed cells OR PLASMA |
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Heinz bodies are made up of? |
Denatured Hemoglobin (DNA) |
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Beta Thalassemia Major is another name for? |
Cooleys Anemia |
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Monosodium urate is highly? |
Birefringent |
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Zygomycota? Geotrichum? |
Sporangium. Arthroconidia. |
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Why is caffeine used in the diazo reaction? |
To measure unconjucated bilirubin |
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How is measurement of iron carried out? |
Step 1: addition of acid step 2: addition of reducing agent step 3: add color reagent |
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Fetal Lung maturity is assessed by examining what parameter? |
Phosphatidylglycerol |
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Protein C what are you taking? |
Warfarin |
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Case study about skin testing? |
T-cells |
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Bilirubin reacts at what fluorescence? |
450nm? |
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N.gonorrheae virulence factor? |
Pili |
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A/A g+ what would you see on HE agar? |
Orange colonies |
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Oral contraceptives are known for causing what? |
Increase in serum FE. |
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What can cause turbidity in synovial fluid? |
Crystals |
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A false positive urobilinogen is caused by? |
Porphobilinogen |
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Caffeine benzoate in bilirubin assay is what? |
Accelerator |
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Preferred specimen for Tacrolimus is? |
Whole Blood
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Preferred testing for legionella? |
Urine antigen testing |
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Test for CMV? |
Viral culture |
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Specimen for whooping cough? |
Nasopharyngeal swab |
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Blood smear picture that looks like Howell bodies, the retic is 18%, the technologist should stain with? |
Howell Jolly stain |
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Disease categorized by unconjugated bilirubin? |
Gilberts Syndrome
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Circulating anticoagulant? |
Heparin
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Fungal stain? |
Cotton blue
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Elevated levels of aminolevulinic acid in the urine is due to presence of? |
Lead
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What is added before doing cell count of synovial fluid? |
Don't know :(. |
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What causes weak D? |
Missing epitope. |
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Walking pneumonia has what effect on penicillin? |
No effect
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what nutrient is incorpriate in anaerobic ager to aid the growth of anaerobes
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Glycerol and Iron |
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Difference between Yersinia pestis and entercolitica? |
Motility |
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Why are first morning sputum needed to diagnose mycobacteria? |
Because if the specimen is NOT first morning it will be invaded.
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What instrument measures fluorescent light and transmits it at a large wavelength? |
Fluorometer |
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Lewis antibodies in saliva of group A? |
Le(a-b+) |
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5HIAA is what? |
Serotonin metabolite. Seen in CARCINOID TUMORS. |
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Stain for cryptosporidium? |
Trichrome |
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Give an example of a non fluorescing bacteria? |
Pseudomonas |
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Clinitest result turns quickly from blue to orange and back to blue….is this test pos or neg
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Negative |
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Electrophoresis SLE? |
CENTROMERE |
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Using compensated polarized light, what is positive (blue) for birefringence?Uric Acid or Calcium pyrophosphate |
Calcium Pyrophosphate |
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when a lab personnel is electrocuted. What to do when he/she is “stuck” to the electric source itself? |
Disconnect the power source. Elevate feet and lower head. |
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Mother Le(a-b-), what would cause this? – |
mother loses lewis antigens while pregnant |
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Bromide affects which electrolyte? |
Potassium |
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Use of heparin manganese? |
HDL precipitation |
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Patient with HA1C of 5%, glucose is 150. |
Patient was following diet for beginning of 3 months and stopped. |
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Bacteria with tapered ends |
Fusobacterium |
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Swarming bacteria, which test should you do next? |
Indole for PROTEUS |
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11) A beta hemolytic gram positive cocci is growing on a blood plate. It is catalase positive, coag negative, oxidase negative, 6.5% NaCl positive.A) Report as normal floraB) Repeat the catalase and report out EnterococcusC) Repeat the coagulase and report out Staph aureusD) Repeat the oxidase and report out Micrococcus |
B Repeat the catalase and report out Enterococcus |
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How long after whole blood donation should platelets be separated from RBCs?How long after whole blood donation should plasma be separated from RBCs? |
5-8 hours |
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There was one question that asked what test hemolysis would effect the least! |
Sodium |
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Indicator of nutrition? |
Prealbumin |
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CSF should be stored at? |
Room Temp |
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DOC SENDS A THROAT SWAB FOR ROTAVIRUS |
CALL FOR CLARIFICATION (SHOULD BE STOOL) |