Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
9 Cards in this Set
- Front
- Back
What is the bacteria that used congo red (a negative stain)
|
Klebsiella pneumoniae Congo Red
|
|
Which Bacteria used manevals stain
|
Staphylococcus epidermidis Maneval’s Stain
|
|
Which Two staining techniques does capsular staining use?
|
Negative stain: using Congo Red to color the background
Simple stain: using Maneval’s stain to color the cell proper of the bacteria |
|
What is a glycocalyx
|
polysaccharide layer is generally referred to as a glycocalyx
|
|
Capsules:
|
the glycocalyx is the polysaccharide containing material surrounding the outside of the
water-soluble is stained normally, will wash away bacteria - heat sensitive or destroy the capsule - composition varies between organisms but they are made of glycoproteins, polypeptides, and polysaccharides - nature (thickness and flexibility) varies also between organisms cel |
|
Two parts to a capsule
|
1. Capsule: rigid layer with a tight matrix; excludes particles like dye
2. Slime layer: easily deformed and usually can’t be seen |
|
Function of capsules
|
1. Increases virulence in some organisms by protecting the cell from phagocytosis by
the immune systembecause antibodies are hidden 2. Prevents dehydration/desiccation due to the attraction of water by the layer 3. Attachment to host cells because of binding reactions between the host tissue and the glycocalyx |
|
Organism Klebsiella Pneumoniae does or does not have a capsule
|
1. Klebsiella pneumoniae => has a capsule and looks like a
figure eight |
|
Staphyloccocus epidermis Does or does not have a capsule
|
2. Staphylococcus epidermidis => has no capsule
|