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37 Cards in this Set
- Front
- Back
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Gram positive cocci in grape like clusters*Non Motile |
Staphylococcus |
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Presumptive test to deffirentiate Staphylococci and Microccus from Streptococci. |
Catalase test (+) |
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Culture media or in the slide (+) results |
Vigorous bubbling |
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Able to grow in 7.5-10% NaCI Resistant to 0.2-0.4 units of bacitracin |
Staphylococci |
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Leofers Serum Slant |
(LSS) |
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Golden yellow Lemon yellow White |
Staphylococcus aureus Staphylococcus citreus Staphylococcus albus |
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Staphylococcus aureus:
-Growth/Colony Morphology |
*Uniform turbidity on broth *On plates they produced smooth circular and opaque colonies with oil-like or butyrous appearance *Jet Black colonies on tellurite agar |
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ENZYMES:
Initiates skin infection. |
LIPASE |
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TEST FOR IDENTIFICATION |
*Catalase (+) *Coagulase (+) *Mannitol Fermentation (+) *DNase test (+) *VP and Nitrate (+) |
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It enhances the invasion into the tissues |
HYALURONIDASE |
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Beta hemolytic |
HEMOLYSIS ON BAP |
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Promote fibrinolysis (dissolution of clot) |
STRAPHYLOKINASE |
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Prevents phagocytosis |
PROTEIN |
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Prevents phagocytosis |
PROTEIN |
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Food poisoning |
ENTEROTOXIN AND A and B |
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causes the Toxic Shock Syndrome |
ENTEROTOXIN F/TSST |
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*Sensitive to Lysotaphin and Novobiocin *Resistant to polymyxin |
SUSCEPTIBILITY TEST |
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*Boils furuncles, carbuncles, folliculitis, cellulitis, wound infection. *Bullous impetigo- skin infection of epiderminds involving epithelial cells. |
Causes: |
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*Food poisoning *Toxic Shock Syndrome *Scalded Skin Syndrome |
TOXIC MEDIATED DISEASES: |
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*Gram (+) cocci in TETRADS strict aerobe (an Oxidizer) *On BAP Gamma Hemolytic |
Micrococcus luteus |
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*Gram (+) cocci in chains spherical to avoid *Non motile Capnophilic (5-10%CO²) *Medium of choice 5% Sheep Blood Agar |
STREPTOCOCCI |
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Incomplete /partial hemolysis on BAP causing the greening of the agar - Streptococcus pnuemoniae, Viridans Streptococci - S mitis and S mutans. |
Alpha hemolytic Streptococci |
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Incomplete /partial hemolysis on BAP causing the greening of the agar - Streptococcus pnuemoniae, Viridans Streptococci - S mitis and S mutans. |
Alpha hemolytic Streptococci |
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Complete hemolysis showing clear zones around the colony. - Streptococcus pyogenes and Streptococcus agalactiae |
BETA HEMOLYTIC STREPTOCOCCI |
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Unable to cause hemolysis on BAP i.e E. Faecalis, E. Faecium S. Bovis |
GAMMA HEMOLYTIC STREPTOCOCCI |
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When a colony is surrounded by an inner alpha and outer beta zone. This my occur due to ptolonged refrigeration. |
ALPHA PRIME |
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Susceptibility test for scarlet fever to determine if the person is at risk of developing the infection (+) result, redness at the site of infection. |
DICK'S TEST |
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Test to determine if the rashes is caused by Scarlet Fever or not injecting anti-erythrogenitic toxin |
SCHULTZ CHARLTON TEST/BLANCHE PHENOMENON |
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Streptococcus agalactiae |
GROUP B STREPTOCOCCI |
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These are the animal pathogens my cause severe pharyngitis followed by bactermia may also cause pneumoniae cellulites and abscess |
GROUP C STREPTOCOCCI |
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*S. Bovis and S. Equines *Growth inhibited in 6.5% NaCI *Penicilin Sensitive Vancomycin Resistant *PYR test (-) Bile Excullin |
GROUP D STREPTOCOCCI (GROUP D NON ENTERCOCCI) S. BOVIS GROUP |
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(E faecalis E. Faecium E. Durans E avium) *Normal GUT, GIT and RT flora common UTI among hospitalize patient *Grows in 6.5% NaCI *Penicillin and Cephalosporin Resistant *Bacitracin Resistant *Bile Esculin and PYR test Positive |
ENTEROCOCCI |
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Presumptive identification of Enterococci and Non Enterococci * Uses 40% bile * Media Bile Esculin Media *Reagent /indicator. Ferric ammonium citrate *Positive result. Blackening |
BILE ESCULIN TEST. |
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For the identification og Pneumococci (+) result if on BAP- clearing of Media In plated media BAP with suspected colonies- add desoxycholate directly to the colonies - incubate at 35-27C in 30 minutes (+) result disintegration/lysis of colonies |
BILE SOLUBILITY TO OPTOCHIN |
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*Alpha- Viridans and S. Pneumoniae *BETA- Group A, B, C ansd some group D (Lancefield) *Gamma- mostly Group D |
HEMOLYTIC PATTERN |
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Known to cause liver, spleen and brain abscesses COLONIES, caramel/butter scroth odor SPECIES. S. Constellatus S. Intermedius S. Anginosus |
MILLERI STREPTOCOCCI COMPLEX |
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*aka thiol-requiring Streptococci/pyridoxal Streptococci Satelliting Streptococci |
Abiotrophia |
