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50 Cards in this Set
- Front
- Back
3 Stages of ESR
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1. rouleaux
2. settling 3. packing |
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What is a Barr body, and where is it found?
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X chromosome precipitate
Neutrophil |
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Describe what happens in the lag phase of the O2 dissociation curve
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Hgb is not able to fully load until one O2 binds
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Nucleus: cytoplasm ration in gran line
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myeloblast- 6:1
promyelocyte- 4:1 myelocyte- 1.5-1 meta- 1:1 Band- 1:2 seg -1:3 |
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Characteristics of myeloblast
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round nucleus and prominant nucleoli
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Lymphopoiesis
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lymphoblast
lymphocyte T cell, B Cell (resting lymph) activated lymph Plasma cell |
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Granulocyte maturation
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myeloblast
promyelocyte myelocyte metamyelocyte band seg |
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Degrees of detection and what found in flow cytometry
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0- size
10- complexity 90- lobularity and granularity |
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Plots %O2 vs. hbg o2 saturation
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o2 dissociation curve
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Way to determine Hgb []
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whole blood -> solution with KCN, KFeCN-> oxidizes heme mlcl.
color intensity measured at 540nm. |
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Drabkins reagent
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KCN & KFeCN
used in hemoglobin determination |
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Causes methemoglobin from hemoglobin
needed for cyanmethemoglobin |
ferricyanide
cyanide |
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Hematocrit determined from____ by
process |
hgb 3X rule
whole blood in microcapillary-> centrifuge, measure %cells:volume |
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Retic stain
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Supravital stain
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Ways to deal with difficult plasma in Hgb measurements
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plasma replacement
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Codocyte aka_
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target cell aka____
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Acanthocyte vs. echinocyte
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irregular and significant vs. poor drying/staining and insignificant (regular)
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Drepanocytes
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Sickle Cells
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increased lipids, bilirubin, WBC (looks like)
Hgb S or C causes |
creamy, yellow, turbid plasma (caused by)
hard to lyse cells |
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Basophilic stippling aka
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punctate basophilia aka
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Papenheimer bodies- erythrocyte
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smaller than Howell jolly bodies.
clumps of Fe (multiple) |
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Howell Jolly bodies- erythrocyte
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DNA remnants- spleen failure
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Induced DIC (activates FX)
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Russell's viper venom
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Fibrinogen testing
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reagent- thrombin
shows how much is left (little= DIC/eclampsia) |
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Factor assay theory
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deficient pt. plasma added to plasma with adhesion of one factor. If no clot= missing pt. factor
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Siderosome
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Papernheimer body- iron
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Fibrin split products & D-Dimer testing is
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quantitative (yes or no) determine break down of clots
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total blood let from George
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3.75 L
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Reagent for PT
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thromboplastin and Ca++ (innovin)
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APTT looks at_____
PT looks at _______ |
intrinsic efficiency test
extrinsic efficiency test |
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Why use elegic acid?
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XII -> XIIa
negative charge |
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ISI
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international sensitivity index
Ideal @ 1 |
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INR calculation
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R^ ISI
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INR based on
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reagents used, method/ instrument, sensitivity
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R (for INR calculation) is
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pt protime/ mean normal population protime
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Von behrens transducer
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avoids impedence with plate
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Test for sickle cell
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osmotic fagility
sickle dex |
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Platelet estimates
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# plts in 30 fields (by tens), average 2 closest (20 fields).
Divide instrument given # by counted #. calibration factor. |
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plt. maturation
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Megakaryoblast
promegakaryocyte megakaryocyte mature plt. |
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PT reagents
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tissue thromboplastin (checks prothrombin)
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exudates vs. transudates
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exudates- infected
transudates- lots of fluid (non infection) |
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What do you dilute cloudy or bloody fluid with?
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cloudy (WBC)- glacialacetic acid
RBC- saline |
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Butt cells
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lymphoma characteristic cell
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keys for neutrophilic toxicity
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toxic granules
left shift vacuoles Dohle bodies |
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Coincidence loss correction
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2 cells through at the same time/ repeated (cycling) of cells
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Volumetric metering
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mercury tells volume
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Wright stain aka______
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Romanowsky stain aka _______
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Wright stain dyes
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polychromatic dye
eosin methaline blue |
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Erythrocyte Line
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pronormal blast
basophilic normoblast polychromatic normoblast orthochromic normoblst polychromatic erythrocyte (retic) RBC |
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last erythrocyte maturation cell to have nucleus
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orthochromic normoblast
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