Histopathology

Father of histology

Father of histopathology and cellular pathology

Propose the use of formaldehyde as a fixative

Recording the tissue specimen in a log book and assigning identification numbers to the received specimen

Preserving the tissue specimen in as life-like a manner as possible

Routine fixative

Removal of calcium from some tissue on organs

Routine decalcifying agent

Removing water from the specimen by using increasing grades of ethyl alchohol

Removing excess alcohol in tissue and makes tissue transparent

Routine clearing agent

Filling up tissue spaces or cavities with melted paraffin wax

Placing the infiltrated tissue inside a mold

Removing excess paraffin wax from the block until it assumes the shape of a truncated pyramid

Cutting of tissue block into thin slices called ribbons/sections using a microtome (commonly used: rotary microtome)

Employs the use of hematoxylin and eosin dyes to differentiate the cells and the cell contituents

Putting the cover slip on the stained tissues using a mounting medium

Specimen number is indicated on the glass slide

To investigate superficial masses or lumps to detect any pathologic condition like malignancy

Insertion of a hollow needle with the mass for sample collection

Screening for cervical cancer and any precancerous changes in the cervix

Also done to detect STDs such as trichomoniasis, candidiasis, and human papilloma virus (HPV)

Paraffin-embedded specimen prepared from dried mucus, sputum, and debris found in body of fluids

To concentrate cells on a slide in uniform monolayer using a high-speed centrifuge

Performed when an immediate or rapid microscopic analysis of specimen is needed cryostat

Biomarkers important in the genetic testing for breast cancer, estrogen receptor, progesterone receptor, Her2-neu, P-53, DNA ploidy analysis

Uses special stains to determine the chemical compounds and their distribution within and in between the biological cells of the body

Detecting antigens in the cells of the tissues sections by using antibodies

Thorough examination of a dead body to determing the cause of death, manner of death, and to evaluate any disease or injury that may have been present