Lab Questions

If a lens is in sharp focus on a specimen and an adjacent lens is swung into position with need for only minor adjustments in the focus, the lens is said to be_________

When a lens is rotated to a higher power, the diameter of the field your eye sees when looking through the microscope is______but the actual size of the specimen as measured on the slide is______

Total magnification of a lens system is calculated by_______the magnification of each lens is the system

The microscope should be stored with the _______lens in the down position

The resolving power of a lens depends directly on the _______of light used

The distance between the specimen and the objective when it is in focus is called the _______p.

Numerical aperture is directly related to the ______ of ______of the glass in a lens

A lens with a pH inscribed on the side would be found on a ________microscope

If the total magnification with 45X high-power objective is 225X, what would be the magnification of the ocular?

The resolution of a microscope is increased by:

Given: Blue light of wavelength 400nm, a condenser, and an objective with a numerical aperture of 1.2. What is the smallest resolvable object in mm diameter seen with such a microscope?

Describe how immersion oil helps in viewing an object.

Ocular- eyepiece

Revolving nosepiece

Objective

Diaphragm

Condenser

Mechanical Stage

Base

Arm

Coarse Adjustment

Fine Adjustment

Body Tube

The small amount of growth on the end of an inoculating loop is called an_______

A flame-sterilized inoculating______ is used to inoculate the medium.

Aseptic technique is important to prevent self-infection and _______ of the culture medium.

In sterilizing your transfer loop, the ______ end most also be sterilized.

Setting the culture cap down on the bench is a violation of ______.

When growth on or in a medium contains only a single species of microorganism, it is called a _______.

A term often used to describe cloudiness in broth media is _______.

When a bacterial culture grows across the surface of a broth, the growth is referred to as a _______.

Cells that settle to the bottom of a broth tube are referred to as ______.

Growth occurring as clumps suspended in the broth is referred to as ________.

Growth around the margin of the surface of a broth tube but not across it is referred to as a _________.

Name one of the ingredients in nutrient broth:

An isolated population of bacteria growing on a solid medium is called a

A slant culture of an organism which is used to make other subcultures is called a ______ culture.

List three factors that might influence the cultural characteristics of an organism growing on an agar medium:

A word meaning "pigmentation" or "color" used to describe growth on an agar slant or colony is _______.

The term used when a culture is placed at a particular temperature for a period of time is ___________.

When an inoculated tube is compared to an uninoculated tube for signs of difference, the uninoculated tube serves as a ______.

Bacterial cells are difficult to see because their index of refraction is very similar to ________

A good smear should appear only slightly_______

A slant culture is generally considered a better source of organisms for staining than is a _______ culture

An important consideration for the slide used for staining is that it can be_______

True or False: Tap water can be used to make smears because there are too few bacteria to see under oil immersion.

A smear is best made if during the heating stage ______ heat is used

True/False: Virtually everyone has bacteria in their saliva.

The number of cells cited in the introduction will result in about 1 cell per oil immersion field when observed under the microscope. If you had ten cells per field, you would have about _______ bacteria per ml of your saliva

The large nucleated cells observed on saliva are most likely ________

If cells stained with methylene blue are washed too long, the dye will be_________

A preparation made by mixing a loopful of water and a bit of agar slant culture on a glass slide is called a _______.

The Gram stain is an example of a ________ stain

All members of the genus Echerichia would be expected to be Gram_______

Endospores usually appear ________ in a completed Gram stain

If your Staphylococcus aureus cells appear pink after Gram staining, the most likely cause was _________.

True/False: Gram-positive and Gram-negative cells have the same color after application of the first two Gram stain reagents.

Generally speaking, endospore-forming bacteria would be Gram-_______.

Gram reactions are reliable only for cultures ____ hours old or younger.

Unless the procedure specifically indicates otherwise, Gram stain smears are first _________.

A smear when properly made should look _______.

The most critical step in making the Gram stain is the application of the ____________.

A culture that is too old may appear Gram-______when stained

Carbolfuchsin stain is prepared by adding ________ to basic fuchin.

The acid-fast stain identifies microorganisms with a high _______content. The primary stain is not washed out by the _________ decolorizer, and the cells are said to be _________.

Members of the genus __________ are usually all acid-fast.

Name two pathogenic acid-fast bacteria and the disease that each one causes

Among bacteria, and acid-fast state is ___________.

The acid-fast decolorizer must not be mistaken for the decolorizer in the _______ stain procedure.

The primary stain in the acid-fast procedure is_________.

When placing mixed cultures on a slide, it is important to _______ the inoculating loop between cultures.

If asepsis is not practiced in the laboratory,________ of cultures will result.

As suggested in the introduction, bacterial growth means an increase in bacteria ________ instead of the size of one cell.

A good aid in determining the margin type of a bacterial colony is a _______ lens.

When you incubate a Petri plate, it should normally be placed in an incubator with the ______ side up.

A mass of cells growing on an agar surface is called a ______.

The mass of cells in question 5 is usually derived from a ______ cell.

Restreaking a colony for isolation several times is necessary to be reasonably sure that you have an ________ _______.

In taking cells from a culture tube for streaking, it is important to ________ the end of the inoculating loop holder before entering the tube.

Streaking a plate with the open face of the loop oriented vertically to the agar surface is a good way to ______ the agar.

Agar should be poured into a plate at a fairly cool temperature to avoid excess ________ on the inside of the lid.

The primary stain in the Schaeffer- Fulton method is _________ ________.

A trace element often used in media to stimulate endospore formation is _______.

Individual species of endospore forming bacterial characteristically form spores that have specific _______, _______, and may swell the cell or not.

The genus _______ is an aerobic sporeforming genus. One species, _______ ________, causes the disease ______.