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26 Cards in this Set
- Front
- Back
The capsule creates a mucoid phenotype. This is Klebsiella pneumoniae
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The appearance of the colonies on this blood agar plate is due to...
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Elek plate. It tests fot toxin presence for C. diphtheriae
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The name of this plate is
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Eosin Methylene Blue (EMB) Agar - is is demonstrating the green metallic sheen of E. coli
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What is the name of this agar and what is the enteric shown growing on it?
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What is an Eosin Methylene Blue (EMB) Agar and what does it contain?
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Contains eosin & methylene blue to inhibit Gram +ve bacteria. Eosin also acts as an indicator for fermentation. Lacotse & sucrose are the sugars
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Which E. coli strain is non-motile, a late/non lactose fermentor and anearobic? and what other species is it very closely related to?
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Enteroinvasive E. coli is non-motile and a late/non lactose fermentor and anaerobic similiar to Shigella
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Which of these enterobacteriacea is NON-motile at 36 degrees? Shigella, Serratia, Proteus or citrobacter?
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Shigella is non motile at 36 degrees. So is klebsiella and Yersinia
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Which enterobacteriaceae are non motile at 36 degrees?
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Shigella, Klebsiella and Yersinia (which is motile at 22 degrees)
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Which of the following is NOT a lactose fermentor? Klebsiella, E. coli, Salmonella, Citrobacter, Serratia?
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Salmonella is non-lactose fermenting. Both serratia and Citropbacter are late lacose fermentors.
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Which enterobacteriaceae species is both motile at 36 degrees AND Voges-Proskaur positive?
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Klebsiella
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Which enterobacteriaceae are hydrogen sulfide positive?
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Salmonella, Proteus, Citrobacter and Edwardsiella
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What category of bacteria ferment glucose, are oxidase negative and (mostly) reduce nitrate to nitrites?
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The Enterobacteriaceae
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Except for Shigella, all clinically relevant enterobacteriaceae are catalase __________
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positive
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What is listeriolysin O?
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It is a virulence factor for listeria monocytogenes which allows it to escape from a phagosome before lysosomal fusion, allowing the bacteria to survive within phagocytes!
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What are the components of the diptheria toxin?
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Fragment A - inhibits protein synthesis
Fragment B - receptor binding/translocation |
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What is the name of the initial lesion casued by cutaneous diptheriae?
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ecthyma diphtheriticum - resembles several other skin conditions
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What are the two forms of C. diphtheriae infection?
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Respiratory and cutaneous
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Both respiratory and cutaneous forms of diptheriae infections can cause what other symptoms?
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Myocardiopathy (20-70%)
Neurological symptoms (20-75%) Fatty/focal necrosis of kidneys, liver or adrenals and death |
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What is the most commonly isolated Corynebacterium species?
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C. jeikeium
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What process was used to isolate L. monocytogenes from placental or other tissue?
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cold enrichment- placed in nutrient broth for months at 3 degrees. Replaced by more rapid id methods
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What growth requirement is specific to C. diphtheriae subspecies intermedius?
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C. diptheria intermedius requires lipids for growth
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C. diphtheriae and C. jeikeium are both urease ____
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negative. Other corynebacterium C. ulcerans and C. pseudotuberculosis are urease positive.
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Which of the following enterobacteriacaea is NOT phenyalanine deaminase positive? Proteus, Pantoea, Morganella, Providencia?
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Pantoea.
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Which of the following is hydrogen sulfide positive? Enterobacter, Serratia, Edwardsiella, Klebsiella, Yersinia?
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Edwardsiella.
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What are the hydrogen sulfide producing enterobacteriacaea?
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Salmonella, Proteus (vulgaris and mirabilis), Citrobacter freundii and Edwardsiella tarda
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This is C. diphtheriae and growth on Loffler medium enhances metachromatic granules. Notice also the "Chinese characters".
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What bacteria is this and what growth medium will enhance the granules seen?
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How does iron affect C. diptheriae toxin production?
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The toxin production is inhibit in high iron environments and enhanced with iron deficiency.
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