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38 Cards in this Set
- Front
- Back
Name this test.
Name the media & reagent used. |
-Starch Hydrolysis test
Media= Starch Agar Reagent= Iodine |
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What are we testing for?
Which one is positive-top or bottom? |
-To see if amylase is present in the bacteria.
-Bottom - Agar around the bacteria is NOT black (has a halo around bacteria) |
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Name this test? Name media. Top or bottom positive?
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-Lipid Hydrolysis
-Spirit Blue Lipid Agar -Top - intense blue around bacteria |
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Name exoenzyme involved. Which one negative and why?
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-Lipase
-Top left - no intense blue around bacteria b/c not breaking down fat b/c doesnt have lipase so negative |
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What is polymer hydrolysis?
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Breakdown of polymer to monomer.
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What's an exoenzyme?
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Enzyme that bacteria produce that break down large molecules into smaller molecules.
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Name Test, media, Exoenzyme & Reagent.
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-Gelatin Hydrolysis
-media= Gelatin Nutrient Agar -Exo= Gelatinase -Reagent= Chloride |
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How does Chloride work in Gelatin Hydrolysis test?
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Precipitates the protein (makes clump up)
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Where is casein found in nature?
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Cow's milk
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Which one is positive and how can you tell?
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Top right- b/c no white around bacteria b/c gelatinase is present and broke down the gelatin in the agar (clear halo around bacteria)
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Name test, media, reagent, and exoenzyme.
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-Casein Hydrolysis
-Skim milk nutrient agar -no reagent -Casease |
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Which one is positive?
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Top left b/c no white around bacteria (Clear halo around bacteria)
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What molecule is hydrolyzed?
Name reagent and media. |
-exonuclease
-no reagent -DNase Agar (Methyl Green) |
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Which result is postivie and why? What does a negative result MEAN?
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No green around bacteria (whiteish tent around bacteria (halo))
-neg result means exonuclease was NOT released |
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What group of organisms is the nuclease test helpful in identifying?
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Food poisoning
(b/c they are thermostable and only nuclease survives) |
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Which one is Alpha, beta and non-hemolytic?
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Name media, exoenzyme & reagent. what does neg result MEAN?
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-blood agar
-hemolysin -no reagent -RBC's are not being brokendown |
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Name media. what does + test look like and what does a - result MEAN?
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-Phenol Red (sugar name) Broth
-+ result is yellow with or without air bubble -neg means not acidic (no fermintation is happening) |
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Name media. Name piece of equipment used to inoculate. What does neg result look like?
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-Kligler Iron Agar
-inoculating needle -Black where bacteria was put |
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Which method uses an organic molecule to make hydrogen sulfide?
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Dissimilation
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Which method uses an inorganic molecule to make hydrogen sulfide?
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Reduction
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What specifically is responsible for forming the color in a positive result?
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Hydrogen sulfide from bacteria reacting to the iron in media
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Name media, reagent and piece of equipment used to inoculate.
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media=SIM media
Reagent=Indole Inoculating needle |
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Which result is positive and what does that mean?
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on right with purple/pink floating. means bacteria produces indole
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Name media, reagent. What's purpose of this test?
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media=MRVP Broth
Reagent= Methyl Red Purpose= if glucose added to bacteria will it make acid. |
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What does V stand for in IMViC?
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Voges Proskauer
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Define Coliform
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Gram negative enteric Bacillus
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Name this test and its purpose.
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Voges Proskauer
to see if bacteria can make 2, 3 butanediol and Acetoin as end products (2gether). |
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Name media & reagent.
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MRVP
VP-A & VP-B |
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What does a positive result look like for this test?
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Red
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Name test & purpose.
Name media & Reagent |
Citrate Test to see if bacteria can use citrate as only source of carbon.
Media= Simmons Citrate Agar No reagent |
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Which result is positive and what does that mean?
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Left (blue) which means that bacteria CAN use citrate as only source of carbon.
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Which 2 IMViC reaction labs use the inoculating needle as equipment vs. the loop?
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Indole and citrate test
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Reagent = hydrogen peroxide
+ is one with bubbles which means that bacteria produces Catalase the bubbles are from catalase breaking down the hydrogen peroxide. |
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Name this test. Name reagent & which result is positive & what it means.
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Oxidase Test
Reagent= Oxidase Reagent + result is blue which means the bacteria produce oxidase which means it contains cytochrome C which means member of ETC & is capable of respiration |
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What gas does durham tube trap in this test?
Name media & Reagents. |
-Nitrogen
-Media=Nitrate Broth -Reagents= Nitrate A Nitrate B Zinc & HCl |
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In the nitrate respiration test if after all possible reagents, the media never changes color, what does this mean for the bacteria?
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Both nitrate respiration and Denitrification occured.
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Name this procedure & media
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Kirby Bauer Method
Media= Mueller Hinton Agar |
