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12 Cards in this Set
- Front
- Back
The GasPak system |
Is useful for culturing anaerobic bacteria on standard microbiological medium because the gas pack generates carbon dioxide and hydrogen |
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The hydrogen will combine with the oxygen present in an anaerobic jar to produce... |
water |
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The gaspak system is the best method for determining... |
..the oxygen requirements of unknown organisms |
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Candle jar |
Useful for culturing organisms which prefer reduced oxygen levels and increased carbon dioxide levels -NOT an oxygen-free system This is the best method of culturing a microaerophile |
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Obligate anaerobe |
A microbe that cannot tolerate oxygen and will be killed in his presence. This type of organism is killed because it lacks the enzymes necessary to eliminate toxic oxygen products. |
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obligate aerobe |
A bacterium that requires atmospheric levels of oxygen for normal growth |
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Facultative anaerobe |
A microorganism that can grow with or without oxygen but usually grows faster (produces more ATP) in its presence |
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Aerotolerant anaerobe |
A microbe which will survive but will not grow in the presence of oxygen. |
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Microaerophile |
An organism which requires reduced levels of oxygen |
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Sodium thioglycollate broth |
Contain substances which chemically combined with oxygen making it unavailable. Anaerobes will grow in this medium. Aerobes can grow but only in the upper layers of this medium |
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In an anaerobic chamber |
A gas pack of chemicals is activated using 10 mL of dh2o (distilled water.) The gas pack releases CO2 an H2 gases. Most anaerobes will prefer elevated CO2 levels. There's a mesh capsule in the lid which contains a catalyst called Palladium,in the presence of this, H2 gas is released from the gas pack and any O2 in the chamber chemically reacts forming H2O. |
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Only obligate anaerobes aerotolerant anaerobes and facultative anaerobes will grow... |
In an anaerobic chamber.Obligate aerobes and microaerophiles will NOT grow Obligate aerobes and microaerophiles will NOT grow Obligate aerobes and microaerophiles will NOT grow NOT grow |