Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
38 Cards in this Set
- Front
- Back
Fixation
|
treatment of tissue with chemical agents (fixatives);
preserves tissues |
|
Fixatives
|
coagulate proteins and make them insoluble
|
|
Dehydration
|
removal of water because embedding agent is immiscible with water
|
|
Clearing
|
treating tissue with agent miscible with melted paraffin eg xylene. Clearing makes tissue transparent
|
|
Embedding
|
making a mold; use of paraffin
|
|
Infiltration
|
completely immersing the tissue in melted paraffin
|
|
Sectioning
|
trimming excess paraffin, or any embedding material, used from tissue- 5micrometers
|
|
Mounting
|
placing sections on slides; could be on adhesive-coated or stained slides
|
|
H&E
|
Hematoxylin and Eosin
|
|
hematoxylin
|
Hematoxylin: Basic stain that preferentially colors acidic components of the cell e.g basophilic components: nucleus; regions rich in ribosomes
|
|
Eosin
|
Acidic dye that stains the compponents of a cell pinkish color. Cytoplasmic components with basic pH stain pink- acidophilic
|
|
Toluidine blue (Tol B1)
|
its molecules polymerize with each other when exposed to high concentrations of polyanions in tissue
|
|
Metachromatic
|
A tissue or component that stains purple (rich in polyanions)
|
|
Metachromasia
|
Toluidine blue exhibits this charateristic: blue stain
|
|
masson's trichome
|
Nuclei=dark blue
Muscle, keratin, cytoplasm= red Mucinogen, collagen= light blue |
|
Staining
|
trimming excess paraffin, or any embedding material, used from tissue- 5micrometers
treating tissues with reagent or dye that makes certain structures visible without affecting others |
|
cryotome
|
rotary microtome in a frozen section environment
|
|
Microtome
|
An instrument used to cut tissue into thin sections for microscpic examination
|
|
Orcein's elastic stain
|
elastic fibers: brown
|
|
Weigert's elastic stain
|
Elastic fibers: blue
|
|
Methamine Silver stain
|
reticular fibers: black, and in polarized light using red plate, crystals they look yellow
|
|
Iron hematoxylin (FeH)
|
muscle striations, nuclei, RBCs: black
|
|
Periodic acid-Schiff (PAS)
|
Glycogen and carb rich molecules: magenta
|
|
Wright's and Geimsa stains (blood cell staining)
|
RBS, eosinophil granules:pink
cytoplasm of monocytes, lymphocytes: blue |
|
Lipochrome
|
intermediate of lipid breakdown
|
|
FITC
|
Fluorescein Isothicyanate: used as a fluorescent label for proteins e.g antibodies
|
|
Hemosiderin
|
Insoluble protein that contains iron
especially found in liver |
|
HRP
|
Horseradish peroxidase;more stable, more popular than AP;
increases detectability of target molecule |
|
AP
|
alkaline phosphatase; dephosphorylates many molecules
|
|
Direct immunocytochemistry
|
antibody labelling; Ab labelled with fluorescent dye, then permitted to react with macromolecule
|
|
Costamere
|
structural component of striated muscle cells; connects sarcomere of muscle to cell membrane
|
|
Resolution
|
the ability to show that two distinct objects are separated by a distance
|
|
Histochemistry
|
method of tissue staining that provides information anout the presence and location of intracellular and extracellular macromolecules
|
|
Autoradiography/radioautography
|
method used for localizing and investigating a specific temporal sequence of events.
uses incorporation of radioactive isotopes into macromolecules, then visualized by the use of an overlay of film emulsion |
|
Indirect Immunocytochemistry
|
Ab prep against 1o Ab. 1o Ab binds antigen, wash unbound, then labelled Ab is alloed to bind complex= 2o complex. viewed via fluorescent microscopy
|
|
Immunocytochemistry
|
ferritin (electron dense molecule) labeling may be used for doth direct and indirect
|
|
Confocal microscopy
|
treat samples with fluorescent dye; laser beam thru specimen causees light emission from dyes; single pts gathered y photomultiplier tube; 3D image produced
|
|
Electron microscopy
|
focusing a beam of electrons; resolution of 0.005nm
|