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8 Cards in this Set
- Front
- Back
What’s the difference between biotechnology & genetic engineering? |
Biotechnology uses microorganisms to make practical products while genetic engineering directly manipulates an organisms genome by using technology |
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3 cycles of PCR |
1. Denaturation 2. Priming 3. Extension |
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Define the denaturation step in PCR |
Exposure to heat separates the 2 strands of the target DNA |
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Define the priming step in PCR |
A mix containing extra DNA primers that provide DNA polymerase w/ the 3’ hudroxyl group required for DNA synthesis |
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Define the Extension step in PCR |
Increases the rate at which DNA polymerase replicates each strand to produce more DNA by increasing temp |
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Describe some applications of biotechnology |
Scientists industrialized natural metabolic reactions of bacteria to make large quantities of acetone, butanol, & antibiotics. |
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Describe some applications of genetic engineering |
1. To eliminate undesirable traits 2. Select for 2 culture cells w/ beneficial traits 3. Combine traits of 2 or more organisms 4. Create organisms that synthesize products |
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What is DNA polymerase derived from hyperthermophillic bacteria use for PCR? |
A thermocycler automatically performa PCR by continuously cycling DNA polymerase derived from hyperthermophillic bacteria through 3 temperature regimes |