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34 Cards in this Set
- Front
- Back
What type of media is Blood Agar?
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differential
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What type of media is Chocolate Agar?
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neither, it's the same as blood agar, except the blood is preheated to allow nutrients like hemin and NAD to escape from erythrocytes.
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What type of stuff is the Chocolate Agar for?
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bacteria w/ very specific nutritional needs, such as H. influenzae
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What type of media is MacConkey Agar?
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selective/ differential
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What kind of stuff grows on MacConkey Agar?
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ONLY gram negatives
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Bacteria that utilize lactose on MacConkey Agar produce what color colonies?
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pink
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Bacteria that are unable to utilize lactose as a sugar source grow what color colonies on the MacConkey Agar?
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clear to brownish
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MacConkey Agar is abbreviated:
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MAC
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Chocolate Agar is abbreviated:
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CHOC
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Blood Agar is abbreviated:
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BAP
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When deciding the appearance of the colonies, you should look for:
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color,
size, shape, elevation |
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Other things to observe about colonies/plate include:
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odors,
hemolysis patterns, discolorations |
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Normal microbial flora are most prevalent where:
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oral cavity,
upper respiratory tract, the colon, the skin, the lower genitourinary tract |
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What body areas are normally free from microbes:
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blood,
cerebrospinal fluid, deep tissues |
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Those bacteria other than normal flora in/on the body are called:
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strict pathogens
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The appearance of the bacterial colonies on a plate is called:
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colony morphology
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Gram stain that binds to peptidoglycan:
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Crystal Violet
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permanently attaches CV to PPG
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Iodine
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removes the CV from the gram negative bacteria, leaving them "invisible"
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alcohol
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the red dye used to stain the gram negatives
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safranin
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Gram positives stain
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purple
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Gram negatives stain
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pink
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The control stain is made up of
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S. aureus and E. coli
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S. aureus is
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G+
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E. coli is
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G-
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The first step to prepare a gram stain is to get the slide ready by:
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Making 2 nickel sized circles on the slide, and labeling one end. Then, flame the inoculating loop to red hot, and briefly allow to cool. Place a very small drop of fresh tap water in a circle, and flame the loop again. Touch the colony gently with the loop and mix with water. Spread the mixture to the edges, and fan w/ hand or paper to allow the smear to air dry. Pick up the slide with a clothespin b/w the circles, and run through the flame 3-4 times. Allow slide to cool briefly.
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The next step is staining with:
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CRYSTAL VIOLET. wait 15 to 30 seconds, then gently rinse with water under the faucet.
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Then, drip ________ solution onto each circle and wait __________.
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IODINE: 15 to 30 seconds, rinse gently with water.
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Add ________ dropwise until the purple dye ___________.
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95% ETHANOL: running, then immediately rinse with water
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Drip ___________onto each circle and wait _________.
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SAFRANIN: 15 to 30 seconds, gently rinse, blot dry on fresh bibulous paper
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These bacteria are ALWAYS catalase +
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staphylococci
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These bacteria are ALWAYS catalase -
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streptococci
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The catalase test uses:
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hydrogen peroxide (H202)
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A positive catalase test is indicated by :
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bubbles!
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